摘要
目的建立检测人或动物血清中类鼻疽伯克霍尔德菌特异性抗体的ELISA检测法,并利用该方法研制用于类鼻疽的诊断的检测试剂盒。方法克隆并表达类鼻疽伯克霍尔德菌的特异性鞭毛抗原,用其作为ELISA检测中的捕获抗原。通过对类鼻疽伯克霍尔德菌感染的家兔血清进行检测来初步证明该方法的可行性。结果间接ELISA方法检测阳性血清效果较好,血清稀释80倍后仍能检测到抗体。结论这是一种比较有效的快速诊断人类鼻疽的方法。
Objective To establish the method for the detection of Burkholderia pseudomallei＇s specific antibody in human or animals serum by ELISA and to apply this in the development of the ELISA detection kit.Methods The specificity antigen of Burkholderia pseudomallei was cloned and expressed in E coli;and it was used as capture antigen in an indirect ELISA method which was developed to detect antibodies in serums.Results The indirect ELISA could detect serum of melioidosis infector which was diluted eighty times.Conclusion The indirect ELISA method is a useful method to detect melioidosis.
出处
《国际检验医学杂志》
CAS
2013年第5期517-518,521,共3页
International Journal of Laboratory Medicine
基金
国家高技术研究发展计划("863"计划)资助项目(2005AA219070)
