摘要
目的探讨血必净(XBJ)对多脏器功能障碍综合征(MODS)炎性因子的影响。方法将90只Wistar大鼠随机分为对照组、模型组与XBJ组。尾静脉注射内毒素(LPS)(15μg/kg)建立MODS模型。观察各组的体征表现,用ELISA方法检测TNF-α、IL-1α、IL-4和IL-10的表达水平。结果①对照组、模型组和XBJ组的死亡率分别为:0、37%和20%;②与模型组相比XBJ可促进了TNF-α的表达,同时抑制IL-1α的表达,且TNF-α和IL-1α高表达集中在6～24 h;③模型组与XBJ组IL-4的表达量在6～12 h与对照组相比明显升高(P<0.05);模型组IL-10在6～48 h与对照组相比明显升高(P<0.05);XBJ组IL-10的表达水平在6 h与对照组相比明显升高(P<0.05)。结论 XBJ能降低LPS介导的大鼠MODS的死亡率,可同时促进TNF-α释放和抑制IL-1α、IL-4和IL-10释放,有效改善MODS的反应状态。
Objective To evaluate the influence of Xuebijing （XBJ） by inflammation cytokines of multiple organ dysfunction syndrome （MODS）. Methods 90 rats were randomly separated into 3 groups, the control group and XBJ group were infused 15 μg/kg LPS in vena caudalis, Then the physical sign appearance was observed and collection the serum to determine the expression concentration of TNF-α IL-1α IL-4 and IL-10 by ELISA in each group at different times. Results （1） The death rate of rats were the percent of 0, 37, and 20 in the control group,model group and XBJ group, respectively. （2） The XBJ enhance the increased amplitude of TNF-α and deduce the increased amplitude of IL-1α by compared with model group, the expression concentration peak of TNF-α and IL-1α is 6 -24 h. （3） The expression concentration of IL-4 in model group and XBJ group in model group has significant ascendant compared with control group at 6 -48 h; XBJ group has significant have significant ascendant compared with control group at 6 - 12 h （p 〈 0.05 ）. The expression concentration of IL-10 ascendant compared with control group at 6h （p 〈 0. 05）. Conclusion The results demonstrated that XBJ could degrade the death rate of MODS induced by LPS,and it could reduce the increased amplitude of TNF-α, whereas enhance the increased amplitude of the serum IL-1α, IL-4 and IL-10, it could also prevent those rats from changing into MODS efficiently.
出处
《中国比较医学杂志》
CAS
2013年第3期26-30,共5页
Chinese Journal of Comparative Medicine
基金
甘肃省自然科学基金(No.1010RJZA052)
关键词
血必净
多脏器功能障碍综合征
死亡率
炎性因子
Xuebijing （XBJ）
Multiple organ dysfunction syndrome （MODS）
Death rate
Inflammatory
