摘要
目的通过抑制zdhhc17基因的表达,探讨其在斑马鱼胚胎发育过程中的作用及机制。方法通过显微注射zdhhc17吗啉反义寡聚核苷酸(MO)抑制zdhhc17翻译,观察斑马鱼胚胎发育过程中相关表型的变化。利用斑马鱼整封原位杂交技术,检测集中延伸运动相关标记基因(dlx3b、ntl、myod1、tbx6等)的表达变化。利用RT-PCR技术,检测集中延伸运动相关调控因子mRNA表达水平的变化。结果敲除zdhhc17基因后,斑马鱼胚胎集中延伸运动出现严重缺陷,主要表现为尾部弯曲变短(82/118),且集中延伸运动相关标记基因表达异常,钙黏附蛋白家族cdh1、padh18a mRNA水平降低。结论 zdhhc17通过调节钙黏附蛋白家族cdh1、padh18a基因的表达,进而影响细胞黏附运动,最终参与调控斑马鱼胚胎集中延伸运动。
Objective To investigate the role of zdhhc17 on embryonic development in zebrafish by loss-of- function experiments. Methods Zdhhc17 antisense morpholino oligonucleotide was injected into embryos to block the translation. The whole mount in situ hybridyzation and RT-PCR were used to detect the abnormal development after knockdown of zdhhclT. Results Knockdown of zdhhc17 impacted convergence and extension movements during zebrafish gastrulation. The tail of zdhhc17 morphants was curved and shorter （82/118）. The expression patterns of dh3b, nil, myndl and tbx6 were affected. The mRNA level of cdhl and padh18a was obviously lower in embryos injected with zdhhc17 morpholino. Conclusion zdhhc17 is critical for zebrafish convergence and extension movements via regulating the expression of cadherin superfamily genes cdhl and pcdh18a and affecting cell adhesion.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第2期259-263,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(31271289)
教育部高等学校博士学科点专项科研基金资助项目(20110131110036)
关键词
zdhhc17
棕榈酰化
集中延伸运动
钙黏附蛋白家族
整封原位杂交
斑马鱼
zdhhc17
Palmitoylation
Convergence and extension movements
Cadherin superfamily
Whole mount in situ hybridization
Zebrafish
