摘要
目的观察慢性氟中毒大鼠肾脏中跨膜转运蛋白多糖（Syndecan）-4和细胞核因子（NF）-KB的表达变化，探讨氟中毒肾损害的发病机制。方法60只SD大鼠按体质量和性别随机分为3组：对照组，小、大剂量染氟组，自由饮用含氟化钠（NaF）〈0．5、10．0、50．0mg／L的自来水，实验期为6个月。采用蛋白印迹方法和实时荧光定量PCR方法检测肾脏Syndecan-4和NF—KB的蛋白和mRNA表达。结果与对照组大鼠肾脏中Syndecan--4蛋白表达水平和NF—KB蛋白表达水平[（100．0±8．1）％、（100．0±10．7）％]相比，小、大剂量染氟组大鼠肾脏的Syndecan-4蛋白表达水平[（198．5±5．6）％、（209．2．4±13．O）％]和NF—KB蛋白表达水平[（284．4±11．1）％、（343．2±2．9）％]均明显升高（P均〈0．05）；小、大剂量染氟组大鼠肾脏中Syndecan-4mRNA表达（0．431±0．058、0．453±O．065）均高于对照组（0．128±0．026，P均〈0．05）。结论慢性氟中毒大鼠肾脏Syndecan-4的表达上调增加肾内NF-KB表达，从而引发肾脏损伤，该过程可能参与慢性氟中毒肾损伤的发生。
Objective Aim of the study is to investigate the expression of syndecan-4 and nuclear factor KB（NF-KB） in the kidney of rat with chronic fluorosis, and to reveal the mechanism of kidney damage resulted from the toxicity of excessive amount of fluoride. Methods According to body mass and sex, sixty SD rats were randomly divided to three groups according to body mass and fed with different contents of fluoride： control group with normal tap-water（ 〈 0.5 mg/L fluoride）, small dosage of fluoride exposure group （adding 10 mg/L fluoride in tap-water） and large dosage of fluoride exposure group（50 mg/L fluoride） for six months. The protein level of syndecan-4 and NF-KB in the kidney was detected by Western blotting and syndecan-4 mRNA level by quantitative real time PCR. Results As compared to the control group[（100.0 ± 8.1）%], the expression ofsyndecan-4 at protein level in the kidney of rat was significantly increased in the small dosage of fluoride exposure group [ （ 198.5± 5.6） %, P 〈 0.05 ] and large dosage of fluoride exposure group [ （209.2 ±13.0）%, P 〈 0.05 ] ; the protein levels of NF-KB in the small dosage of fluoride exposure group[ （284.4 ± 11.1）%, P 〈 0.05] and in the large dosage of fluoride exposure group [ （343.2 ± 2.9）%, P 〈 0.05 ] were significantly increased than that of the control group [ （100.0 ± 10.7）% ]. The mRNA levels of syndecan-4 in the kidney in the small dosage of fluoride exposure group and large dosage of fluoride exposure group（0.431 ± 0.058 and 0.453 ±0.065, both P 〈 0.05, respectively） were significantly increased than that of the control（0.128 ± 0.026）. Conclusions The increased expression of NF-KB in the kidney is induced by increased expression of syndecan-4, which may be involved in kidney damage of chronic fluorosis.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2013年第2期133-135,共3页
Chinese Journal of Endemiology
基金
国家自然科学基金（81160335）
国际科技合作项目（2010DFB30530）
科技部支撑计划课题（2011BAZ03220）
贵州省科学技术基金项目（黔科合J字[201212040号）、贵州省国际科技合作项目（黔科合外G字[2011]7014号）
