摘要
目的评价薯蓣皂苷对大鼠胶原性关节炎(collegeninduced arthritis,CIA)的治疗作用并探讨其可能的作用机制。方法于大鼠左后足底皮内注射胶原乳剂制备CIA模型造模两周后灌胃给药14 d,每4天测量1次右后足跖容积。处死后取右后足作病理切片。Western blot法测定踝关节滑膜组织中NF-κBp65亚基和环氧化酶-2蛋白表达。用放射免疫法检测了足爪TNF-α及PGE2含量。结果薯蓣皂苷可抑制CIA大鼠的足趾肿胀,与模型相比差异有显著性;降低足爪TNF-α及PGE2含量;明显降低NF-κBp65亚基和COX-2的水平。结论薯蓣皂苷对CIA大鼠具有较强的抗炎作用,机制可能与抑制NF-κBp65亚基和COX-2的表达有关。
Aim To investigate the protective effect of dioscin on rats with collagen-induced arthritis（CIA） and its possible mechanism.Methods The models were made by intradermal injecting chicken type II collagen on the left toes of rats,then each group was given the corresponding medicine after modeling 2 weeks for 14 days.the right hind paw volume of rats was determined every 4 days.Rats were killed and the right hind paw was taken to make pathological section.The expressions of NF-κBp65 subunit and COX-2 in rats’ synovium of ankle joint were determined by Western blot method.The contents of TNF-α and PGE 2 in claw were detected by the radioimmunoassay.Results Dioscin could inhibit the swelling of digitipedis of rats with collagen arthritis.There was significant difference between the dioscin groups and model group.Dioscin could lower the levels of NF-κBp65 subunit and COX-2 in rats’synovium of ankle joint and the contents of TNF-α and PGE 2.Conclusion Dioscin can inhibit the inflammatory reaction of collagen arthritis rats.Inhibiting the expression of NF-κBp65 subunit and COX2 may be part of its mechanism.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2012年第10期1464-1467,共4页
Chinese Pharmacological Bulletin
