摘要
马铃薯晚疫病菌（Ｐｈｖｔｏｐｈｔｈｏｒａ ｉｎｆｅｓｔａｎｓ）在我国能够进行有性生殖产生卵孢子［１］，研究了离体培养时培养基成份、温度、光照及ｐＨ值对卵孢子产生的影响。结果表明：白云豆培养基、小米培养基和大豆培养基均适合卵孢子的产生，产生的数量为１３１．６～１４９．６个／ｃｍ２，但冰冻豌豆培养基不适合卵孢子的产生，产生的数量仅为５．８个／ｃｍ２。比较不同ｐＨ值、不同温度、不同光照条件对卵孢子产生的影响发现，ｐＨ值为７、温度为１８℃，黑暗培养最适合卵孢子的产生，强酸或强碱的环境对卵孢子产生均不利。在日光灯或近紫外光光照１２ｈ与１２ｈ黑暗交替情况下培养也不能产生卵孢子。
This paper dealt with the influences of different media, temperature, light and pH to the amount of oospore formation. The results showed that millet agar, bai yun beau agar and soybean agar provided the favorable condition for oospore formation, the number of oospores was 131.6~149.6 /cm2, but a few oospores was formation on frozen pea agar. The results also suggested that the optimal conditions for oospore formation were 18℃, pH7 and darkness. The oospores failed to be formed tinder fluorescent or black light.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第5期317-320,共4页
Microbiology China
基金
河北省自然科学基金资助项目
