摘要
在 36只麻醉Sprague Dawley大鼠 ,观察了最后区内微量注射辣椒素 (10 μmol/L ,5 0nl)对平均动脉压(MAP)、心率 (HR)和肾交感神经放电 (RSNA)的影响。实验结果如下 :(1)最后区内注射辣椒素可引起MAP、HR和RSNA明显增加 ,分别由 12 34± 0 5 3kPa、32 8 5 2± 7 5 4bpm和 10 0± 0 %增至 15 17± 0 2 5kPa (P <0 0 0 1)、35 4 81± 8 5 4bpm (P <0 0 0 1)和 15 6 95± 7 5 7% (P <0 0 0 1) ;(2 )静脉注射辣椒素受体阻断剂钌红 (10 0mmol/L ,0 2ml)后 ,辣椒素的上述效应可被明显抑制 ;(3)预先应用NMDA受体阻断剂MK 80 1(5 0 0 μg/kg,0 2ml,iv)也明显抑制辣椒素的兴奋效应。以上结果提示 ,最后区微量注射辣椒素对血压、心率和肾交感神经放电有兴奋作用 。
WT5”BZ] The effects of capsaicin microinjection into area postrema (AP) on mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA) were investigated in 36 anesthetized Sprague Dawley rats. The results obtained are as follows. (1) Following microinjection of capsaicin (10 μmol/L, 50 nl) into the AP, MAP, HR and RSNA were significantly increased from 12 34±0 53 kPa, 328 52±7 54 bpm and 100±0% to 15 17±0 25 kPa ( P <0 001), 354 81±8 54 bpm ( P <0 001) and 156 95±7 57% ( P <0 001), respectively. (2) Ruthenium red (RR, 100 mmol/L, 0 2 ml, iv), a capsaicin receptor antagonist, significantly inhibited these effects of capsaicin. (3) Pretreatment with a NMDA receptor antagonist MK 801 (500 μg/kg, 0 2 ml, iv) also reduced these effects of capsaicin. The above results indicate that microinjection of capsaicin into AP induces excitatory effects on MAP, HR and RSNA, which are mediated by capsaicin receptors with glutamate involvement.
出处
《生理学报》
CAS
CSCD
北大核心
2000年第5期435-439,共5页
Acta Physiologica Sinica
关键词
辣椒素
最后区
平均动脉压
心率
肾交感神经活动
钌红
MK-801
capsaicin
area postrema
mean arterial pressure
heart rate
renal sympathetic nerve activity
ruthenium red
MK-801
