摘要
目的:研究大黄素与乌头碱配伍对原代培养正常小鼠乳鼠结肠Cajal间质细胞(ICC)生物膜的影响。方法:采用酶解消化法原代结肠间质细胞培养技术,分别将浓度为1,0.3,0.08 mg.L-1的大黄素高、中、低剂量药效浓度与1 g.L-1乌头碱配伍作用于昆明种小鼠乳鼠结肠间质细胞30 min,测定其对细胞丙二醛(MDA)含量、酸性磷酸酶(ACP)活力的影响。结果:1 g.L-1乌头碱单独作用30 min后,与正常组细胞相比,其MDA含量(0.313±0.019 nmol.mL-1和ACP(386.63±57.89)U.g-1活力增加(P<0.01);大黄素高、中剂量药效浓度分别与乌头碱配伍作用后,相对于乌头碱单独作用组,细胞介质中MDA含量[(0.299±0.029),(0.280±0.024)nmol.mL-1]和细胞中ACP活力[(264.31±37.21),(312.23±46.86)U.g-1]有所降低(P<0.05),但仍高于正常组细胞。结论:大黄素与乌头碱配伍后能降低细胞膜的脂质过氧化损伤程度,且大黄素能减轻乌头碱对ICC溶酶体膜的损伤作用。
Objective： To investigate influences of compatibility of emodin and aconitine on the biomembrane of in vitro cultured interstitial cells of Cajal （ICC） from murine conlon. Method： ICCs of kunming mice＇ s conlon were obtained by enzyme-digestion and culture in vitro. ICCs were cultured with compatibility of emodin （ 1, 0. 3, 0.08 mg .L-1 ） and aconitine （ 1 g ·L-1 ） to study the changes of malondialdehyde （MDA） and acid phosphatase （ACP）. Result： Aconitine could increase the content of MDA and the activity of ACP in the cells （P 〈 0.01 ）. The compatibility of emodin and aconitine could reduce the content of MDA and the activity of ACP in the ceils （P 〈 0. 05 ）. Conclusion ： Emodin can lighten the cell trauma of lysosomal membrane witch caused by aconitine. The compatibility of emodin and aconitine can protect the cell biomembrane.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第7期189-192,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑计划重点项目(2009BA184B04)
国家"十二五"科技支撑计划(2010BAE00406)
重大新药创制科技重大专项(2013ZX09201-018)
