摘要
为探索基因rpfG在产酶溶杆菌中的功能,以产酶溶杆菌OH11为研究对象,在基因组测序的基础上,通过同源比对,在OH11基因组中鉴定出rpfG的同源基因,并命名为rpfGLys。利用同源重组技术,对rpfG基因进行了定向敲除,获得了rpfG的缺失突变株。与野生型相比,rpfG基因的突变降低了产酶溶杆菌重要抗菌物质———热稳定抗菌因子HSAF的产量和对腐霉的拮抗活性。荧光定量PCR显示,在rpfG突变株中负责生物合成HSAF的关键基因Lysegl002651的表达显著下调,与HSAF活性检测结果相吻合。然而,rpfG的突变不改变产酶溶杆菌4种胞外抗菌水解酶的活性。另外,rpfG突变导致产酶溶杆菌菌落表面干燥,但滑行能力提高。
To analyse the function of rpfG in Lysobacter enzymogenes OH11, a paralogous gene of rpfG was identified through sequence homology analysis and named as rpfGlys. With homologous recombination technology, a deletion mutant of rpfG was generated. Compared with the wild type, the mutant had a low secretion of heat stable antibacterial factor （ HSAF ） which is an important antibacterial substance of Lysobacter enzymogenes and a lower antipathogenic activity toward Pythium aphanidermatum. Fluorogenic quantitative PCR showed that expression of the key gene（ Lysegl002651 ） which is in charge of biosynthesis of HSAF was significantly lower,which coincided with activity detection of HSAF. Further researches found that the mutant did not change the activities of four- type lyric enzymenes of Lysobacter enzymogenes. Moreover, it was of interest that although mutation of rpfG led to acrid surface of colonies, its sliding ability was strengthened.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2013年第2期45-50,共6页
Journal of Nanjing Agricultural University
基金
国家公益性行业(农业)科研专项(200903052
201003004
201203034)
国家863计划项目(2011AA10A205)
国家自然科学基金项目(31101478)
教育部高校博士点基金(20100097120013)
现代农业产业技术体系(nycytx-29-09)
农业部948项目(2010-C18)
