摘要
以潮棕壤为研究对象,探讨了土壤浸提液冷冻干燥过程中调节pH、添加连二亚硫酸盐缓冲液(BD,0.11 mol L-1NaHCO3+0.11 mol L-1Na2S2O4)及二者组合对31P核磁共振(NMR)谱图分辨率的影响,试验设置对照CK、pH、BD、pH+BD添加等4个处理。结果发现,与对照和pH+BD处理相比,pH和BD单独处理31P NMR谱图分辨率更好。pH和BD单独处理比较,pH处理使谱图化学位移发生了较大的偏移,影响到土壤磷化合物的确定,而BD处理对谱图化学位移的影响较小。添加BD处理中,正磷酸盐、焦磷酸盐、多磷酸盐、单酯、磷脂、DNA等磷组分均可被检测出,而其他处理仅能检测出正磷酸盐、焦磷酸盐和单酯。总之,添加BD处理比其他处理较好地降低了谱图的化学位移偏移,提高了谱图分辨率,并检测出了较多的土壤磷组分。
In order to determine the resolution of 31p nuclear magnetic resonance （NMR） spectroscopy influenced by pH adjustment, buffered dithionite （BD, 0.11 mol L-1 NaHCO3 ＋ 0.11 mol L-1 Na2S204） addition and their combination during soil extraction lyophilization, aquic brown soil was extracted by NaOH-EDTA and four treatments were designed, i.e. CK, pH, BD and pH＋BD. Results showed that the resolution of 31p NMR spectroscopy was better under pH and BD treatments than CK and pH＋BD treatments. Compared with BD treatment, chemical shift changed greater under pH treatment. Soil P composition such as orthophosphate, pyrophosphate, monoesters, phosphonates, polyphosphate and DNA were detectable under BD treatment, while only orthophosphates, pyrophosphate and monoesters were detectable under the other three treatments. Resolution of 31p NMR spectroscopy performed best under BD treatment, as chemical shift was almost not influenced and more P compositions could be detected .
出处
《土壤通报》
CAS
CSCD
北大核心
2013年第2期328-332,共5页
Chinese Journal of Soil Science
基金
国家重点基础研究发展计划(“973”计划)项目(2011CB100504)资助
