摘要
文章研究了宫颈癌细胞经不同剂量的电子线辐照后的红外光谱,比较放射源各剂量组光谱间的差异,分析了不同剂量的射线对细胞造成的辐射损伤情况。结果表明:3413cm-1在9Gy剂量组波数减小最明显,使其N-H基团氢键化;1542cm-1谱带普遍红移,蛋白质分子间的氢键遭到破坏,构象变得疏松无序,使吸收峰向低波数移动;1398cm-1向低波数频移,电子线以碰撞损失能量的方式作用于磷脂分子,改变膜脂中磷脂分子的排列状态,造成其对应红外光谱频率向低波数移动的现象;研究结果得到了电子射线辐照宫颈癌细胞的较佳放射剂量,为研究宫颈癌治疗效果及临床拟定宫颈癌的放疗剂量提供实验依据和参考。
This article studies the IR of cervical cancer cells being irradiated by electronic line of different dose, contrasts with the IR differences of electronic line in each dose group, analyzes the damages from different radiation. Results shows： 3413cm^-1 band of cervical cancer ceils decreased most obviously in 9Gy dose group, then N -H base hydrogen bonding degree increases. 1542cm^- 1 has red shift in common, hydrogen bond among the protein molecule is destroyed, protein form changes into loose moves to low wave number , electronic line crashes molecules in membrane lipid changes because of the disorder, that makes the 1542cm＆-1 has red shift. 1398cm＆-1 the phospholipid molecules, the arrangements of phospholipid lost energy, so the IR corresponding to 1398cm^-1 moves to low wave number. So we finds： the best radiation dose which can damage the cervical cancer ceils by electronic line, and provides experimental basis and reference for clinical radiotherapy dosage.
出处
《河南机电高等专科学校学报》
CAS
2012年第5期22-25,共4页
Journal of Henan Mechanical and Electrical Engineering College
关键词
电子线
光谱手段
剂量
宫颈癌细胞
红外光谱
electronic line
spectroscopic method
dose
human cervical cancer cells
Infrared spectrum （IR）
