摘要
为了掌握植物叶片中花色素苷含量对植物叶片PSⅡ功能的影响情况,以土庄绣线菊Spiraea pubescens、金焰绣线菊S.×bunmalba cv.Goldflame和金山绣线菊S.×bunmalba cv.Goldmound为试验材料,利用叶绿素荧光技术研究了花色素苷在绣线菊叶片光合机构中的作用。结果表明:三种绣线菊的完全展开叶片(即成熟叶片)中的花色素苷含量较低,叶绿素含量以及叶绿素荧光参数之间的差异较小;而在三种绣线菊的伸展叶片(生长叶片)中,金焰绣线菊和金山绣线菊叶片中花色素苷含量明显高于土庄绣线菊,而其叶绿素含量却明显低于土庄绣线菊。金焰绣线菊和金山绣线菊伸展叶片的初始荧光(Fo)、最大荧光(Fm)和单位反应中心吸收的光能(ABS/RC)均明显低于土庄绣线菊,说明较高含量的花色素苷降低了绣线菊叶片对光能的吸收,但是PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在光化学活性(Fv/Fo)、电子传递速率(ETR)和实际光化学效率(ФPSⅡ)却明显高于土庄绣线菊。土庄绣线菊伸展叶片的有活性的PSⅡ反应中心数量明显低于金焰绣线菊和金山绣线菊,而单位反应中心吸收的光能(ABS/RC)、单位反应中心捕获的用于还原QA的能量(TRo/RC)、单位反应中心捕获的用于电子传递的能量(ETo/RC)和单位反应中心耗散掉的能量(DIo/RC)均明显高于金焰和金山绣线菊,且土庄绣线菊伸展叶片失活反应中心的比例较高。花色素苷不但改变了金焰绣线菊和金山绣线菊伸展叶片对光能的吸收,而且改变了光合电子的传递速率和能量分配参数,影响了绣线菊伸展叶片的PSⅡ功能。
In order to learn effects of anthocyanins content on PS II function in leaf of plant, taking Spiraea pubescens, S. ×bunmalba cv. Goldflame and S. ×bunmalba cv. Goldmound as materials, function of anthocyanins in photosynthetic apparatus in leaf was investigated by using the chlorophyll fluorescence technology. The results show that anthocyanin contents are lower in mature leaves of three varieties than in expanding leaves, chlorophyll contents and chlorophyll fluorescence parameters have no significant difference among the three varieties. However, anthocyanin contents in expanding leaves ofS. xbunmalba cv. Goldflame and S. xbunmalba cv. Goldmound are obviously higher than that of S. pubescensl but chlorophyll contents are obviously lower than that of S. pubescens. Meanwhile, minimal fluorescence （Fo）, maximum fluorescence （Fro） and absorption light energy per reaction center （ABS/RC） in expanding leaves ofS. xbunmalba cv. Goldflame and S. ×bunmalba cv. Goldmound are obviously lower than that of S. pubescens, which indicates that anthocyanins can reduce absorption light energy in Spiraea leaves. Furthermore, the parameters of PS Ⅱ photochemical efficiency （FJFm）, PS II potential photochemical activities （Fv/Fo）, electron transport rate （ETR） and actual photochemical efficiency （φPS Ⅱ ） in expanding leaves of the others are obviously higher than that of S. pubescens, respectively. The number of PS Ⅱ reactive center, absorption light energy per reaction center （ABS/RC）, trapped light energy for reducing QA per reaction center （TRo/RC）, trapped light energy for electron transport per reaction center （ET/RC） and dissipated light energy per reaction center （DIo/RC） in expanding leaves ofS. pubescens are significant higher than the others, whilethe proportion of inactivation of reactive center in S. pubescens young leaves was higher than others. Anthocyanins not only changes absorption of light energy in expanding leaves of S. ×bunmalba cv. Goldflame and S. ×bunma
出处
《经济林研究》
北大核心
2012年第4期19-26,共8页
Non-wood Forest Research
基金
国家科技支撑项目(2011BAD08B02-3)
国家自然科学基金项目(31070307
30771746)
黑龙江省自然科学基金重点项目(ZD201105)
黑龙江省重大项目(GA09B201-02)
关键词
绣线菊
花色素苷
PSⅡ
叶绿素荧光
Spiraea
anthocyanin
photosystem II
chlorophyll fluorescence
