摘要
目的:探讨子痫前期(PE)患者血浆对人脐静脉内皮细胞(HUVEC)增殖及溶血磷脂酸(LPA)受体蛋白Edg4表达的影响。方法:体外培养HUVEC,取对数生长期的细胞分为对照组、正常晚孕组、轻度PE组和重度PE组4组,分别用RPMI1640培养液、含体积分数20%正常晚期孕妇血浆、20%轻度PE患者血浆和20%重度PE患者血浆的RPMI1640培养液继续孵育48h。应用MTT比色法检测细胞增殖能力,应用免疫细胞化学SP法和RT-PCR检测细胞Edg4蛋白及其mRNA表达水平。结果:4组细胞增殖抑制率及Edy4蛋白、mRNA的表达差异均有统计学意义(F=104.200,χ2=32.941,F=5803.360,P均<0.05)。与对照组和正常晚孕组相比,轻度和重度PE组HUVEC增殖抑制率增大,Edg4蛋白及mRNA表达明显升高,且重度PE组较轻度PE组升高更明显(P<0.05)。结论:PE患者血浆LPA水平升高可能是引起血管内皮细胞损伤的原因之一。
Aim：To investigate the influence of pre-eclampsia（PE） patients’ plasma on proliferation and lysophosphatidic acids receptor Edg4 expression of human umbilical vein endothelial cells（HUVECs）.Methods：HUVECs were allocated into control group,the normal late pregnant group,the mild PE group and the severe PE group.Cell proliferation was measured by MTT assay,and immunohistochemistry（SP method） and RT-PCR were used to measure the protein and mRNA expressions of Edg4.Results：There were significant differences in cell proliferation inhibition rate,and the expressions of Edg4 mRNA and protein among the 4 groups（F=104.200,χ2=32.941,F=5 803.360,P0.05）.Compared with those of the control group and the normal late pregnant group,the cell proliferation inhibition rate,the levels of Edg4 mRNA and protein expressions in PE group were significantly higher,especially in severe PE group（P0.05）.Conclusion：Lysophospholipids acid may be one of the reasons for endothelial cells damage in PE patients.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第1期96-99,共4页
Journal of Zhengzhou University(Medical Sciences)
关键词
子痫前期
溶血磷脂酸
Edg4
内皮细胞损伤
pre-eclampsia
lysophosphatidic acid
endothelial differentiation gene-4
endothelial cell damage
