摘要
目的 建立肠道病毒 (EVs)检测方法 ,探讨EVs感染与心肌损伤的关系。方法 用套式逆转录聚合酶链反应 (nPCR)方法 ,对 2 2 5例临床拟诊病毒性心肌炎 (VMC)患者血白细胞中EVs RNA进行检测 ,并用ELISA方法检测患者血清心肌肌钙蛋白I(cTnI)。结果 46例EVs RNA阳性 ,检出率为 2 0 4% ;13例正常对照均阴性。EVs RNA阳性患者血清cTnI水平 (13 3± 12 3ng/ml)略高于EVs RNA阴性患者血清cTnI水平 (11 6± 13 7ng/ml)。 14例EVs RNA阳性患者随访 4个月 ,血清cTnI水平从 19 7± 9 8ng/ml增至 32 0± 8 5ng/ml,呈明显增高趋势。 结论 nPCR方法是检测EVs感染的可靠方法。EVs RNA的存在可能是心肌炎不良愈后的指标之一。
Objective To develop a method through molecular biology for having diagnostic evidence of enteroviral RNA from biological samples.Methods The method of nested polymerase chain reaction (nPCR) was used to determine enteroviral RNA in white blood cell from patients with a tentative diagnosis of viral myocarditis (VMC) and normal individuals.Serum cardiac troponin I(cTnI) was also detected by ELISA.Results Positive enteroviral RNA was obtained in 46 of 225(20 4%)patients and none in normal controls.cTnI as slightly higher in EVs positive patients than that in EVs negative patients.Afler four months follow up,cTnI tended to increase significantly from 19 7±9 8ng/ml to 32 0±8 5ng/ml in 14 patients that were enteroviral RNA positive.Conclusion The results suggest that nPCR is a useful method for diagnosing VMC.The presence of enteroviral RNA may be an adverse prognostic indicator of clinical outcome in patients with myocarditis.
出处
《江苏医药》
CAS
CSCD
2000年第10期755-757,共3页
Jiangsu Medical Journal
基金
国家"九五"攻关基金!(96 9060213)
江苏省科委社会发展基金!(BS970 2 8与BS980 39)
江苏省卫生厅项目!(H970 1)
