摘要
目的 观察慢性肝病患者肝脏卵圆细胞的形态学特征 ,并探讨卵圆细胞数量和肝纤维化分级的相关性。方法 以免疫组织化学染色法在 3例正常人和 2 9例慢性肝病患者的肝脏切片中寻找卵圆细胞。对符合卵圆细胞的形态学特征并呈胞浆阳性染色的细胞进行计数。结果 正常肝脏组织中未见到卵圆细胞。在慢性肝病患者肝脏组织中 ,卵圆细胞主要存在于汇管区和纤维隔内 ,以细胞核呈卵圆形、细胞体积小和胞浆量少为特征。 0～ 4期肝纤维化组的卵圆细胞数分别为 7± 3 ,12± 3,2 5± 7,33± 9和 44± 10 ,方差分析表明 ,随肝纤维化程度的加重 ,卵圆细胞数显著增高 (F =2 2 .6 0 ,P <0 .0 1)。除0～ 1和 1～ 2期肝纤维化组之间外 ,各组之间该细胞数差异均有显著性 (P值均分别 <0 .0 5 )。卵圆细胞数与肝纤维化分级有显著相关性 (r =0 .88,P <0 .0 1)。结论 在慢性肝病患者的肝脏组织中 ,常可见到卵圆细胞。
Objective To observe the morphological characteristics of oval cells in human chronic liver diseases, and determine whether there is a relationship between the number of oval cells and liver fibrotic stage. Methods Oval cells were detected in paraffin bedded liver sections of 3 normal subjects (as controls) and 29 chronic liver diseases, using histoimmunochemistry. Cells were counted if they fulfiled the morphological criteria for oval cells and showed cytoplasmic staining. Results Oval cells were not observed in normal livers. In chronic liver diseases, oval cells were located predominantly in the periportal region and fibrosis septa, characterized by an ovoid nucleus, small size, and scant cytoplasmic. The number of oval cells increased significantly ( F=22.60, P <0.01) as fibrosis stage increased in chronic liver diseases (7±3,12±3,25±7,33±9 and 44±10 in 0～4 fibrosis stages, respectively). There were significant differences between any two groups ( P <0.05) except between 0～1 and 1～2 fibrosis stage groups. The number of oval cells was significantly associated with liver fibrosis stage ( r=0.88, P <0.01). Conclusion Oval cells are frequently detected in liver of patients with chronic liver diseases. There is an association between the fibrosis stage and increasing number of oval cells.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2000年第5期301-303,共3页
Chinese Journal of Digestion
关键词
卵圆细胞
慢性肝病
肝纤维化
肝硬化
病理学
Oval cell
Chronic liver disease
Liver fibrosis
Hepatic cirrhosis
