摘要
目的探讨山茱萸多糖对人宫颈癌HeLa细胞体外增殖的抑制作用及对相关凋亡抑制蛋白Survivin和Bcl-2表达的影响。方法采用MTT法、流式细胞术及免疫组化SP法检测山茱萸多糖对HeLa细胞抑制作用及Survivin和Bcl-2蛋白表达的变化。结果山茱萸多糖对HeLa细胞抑制作用明显,抑制程度与浓度、时间相关;6.25g/L、12.5g/L、25g/L的山茱萸多糖对HeLa细胞的凋亡率分别为10.1%、21.6%和28.9%,明显高于对照组的1.2%。山茱萸多糖致使HeLa细胞凋亡抑制蛋白Survivin和Bcl-2的表达量降低。结论山茱萸多糖对HeLa细胞增殖有较强的抑制作用,能够降低Survivin和Bcl-2的表达,诱导细胞凋亡。
Objective To explore the inhibitory effect of fructus corni polysaccharides on human cervical cancer cells and expressions of Survivin and Bcl-2 in HeLa cells. Methods MTT assay was used to determine the cell proliferation. Flow cytometry and immunohistochemistry SP methods were used to detect the apoptosis and protein expressions of Survivin and Bcl-2 in HeLa cells. Results There was a significant inhibitory effect of fructus corni polysaccharides on the prolifera- tion in HeLa cells. The inhibitory degree was correlated with different concentrations and different action times of fructus cor- nipolysaccharides. The apoptosis rates of HeLa cells were 10.1%, 21.6% and 28.9% for fructus corni polysaccharides concentrations of 6.25 g/L, 12.5 g/L and 25 g/L. The expressions of Survivin and Bcl-2 in HeLa cells were significantly lower in fructus corni polysaccharides treatment group than those of control group. Conclusion Fructus corni polysaccharides showed significant inhibitory effects on the proliferation in HeLa cells, which also decreased Survivin and Bcl-2 expressions and induced the cell apoptosis.
出处
《天津医药》
CAS
北大核心
2013年第3期197-199,共3页
Tianjin Medical Journal
基金
河北省人口和计划生育委员会科研计划项目(项目编号:2009-B18)
保定市科技局科学技术研究与发展指导计划项目(项目编号:09ZF011)
关键词
山茱萸
多糖类
宫颈肿瘤
HELA细胞
微管相关蛋白质类
基因
bcl-2
细胞凋亡
CORNUS OFFICINALIS
polysaccharides
uterine cervical neoplasms
HeLa cells
microtubule-asso-ciated proteins
genes, bcl-2
apoptosis
