摘要
目的探讨川穹嗪对实验性急性心肌梗死(AMI)大鼠心肌的保护作用及机制,为其临床应用提供理论依据。方法将40只成年Wistar大鼠随机分为对照组(n=10)、模型组(n=15)及治疗组(n=15),后两组采用结扎左前降支的方法制备AMI模型;对照组行假手术(不结扎冠状动脉)。造模后24 h治疗组腹腔注射川穹嗪120mg/kg,1次/d,连续4周;另两组不干预。术后4周行多普勒心脏彩超检查测定心功能指标,检测血清学指标;处死大鼠称取心脏质量。结果模型组左室舒张末期内径(LVDd)、左室收缩末期内径(LVDs)和E/A值均高于对照组,射血分数(EF)、短轴缩短率(FS)均低于对照组(P均<0.05)。治疗组LVDd、LVDs和E/A值均低于模型组,EF、FS均高于模型组(P均<0.05);VEGF、过氧化物歧化酶(SOD)水平均高于模型组(P均<0.05)。模型组及治疗组EF均与VEGF、LDH水平有显著相关性。结论川穹嗪对AMI大鼠心肌具有保护作用,其机制可能与增强心肌细胞清除氧自由基能力和促进血管生成有关。
Objective To investigate the protective effect of tetramethylprazine on myocardium in experimental myo- cardial infarction rats and its mechanism. Methods A total of 40 Wistar rats were involved in this study. The living rats were randomly divided into three groups, which were control group （n = 10）, acute myocardial infarction group （n = 15 ） and tetramethylprazine group（ n = 15 ）. Acute myocardial infarction model was established by ligating left anterior descend- ing artery of rats. Rats in tetramethylprazine group were injected with tetramethylprazine by means of lumbar injection at 120 mg/kg perday and lasted for four weeks. The control group experienced the same procedure with acute myocardial in- farction group in operation, but with no ligation of coronary artery. Four weeks after operation, doppler echocardiography and serological examination were used to test cardiac function in each group. All rats were killed to record their body weight and heart weight. Results The left ventrieular end-diastolic dimension （LVDd）, left ventricular end-systolic dimension （LVDs） and E/A ratio of acute myocardial infarction group were higher than those of control group, while ejection faction （EF） and fractional shortening （FS） were lower than those of control group （ all P 〈 0.05 ）. LVDd, LVDs and E/A ratio in tetramethylprazine group were lower than those of acute myocardial infarction group, while EF and FS were higher than a- cute myocardial infarction group （ all P 〈 O. 05 ）. Vascular endothelial growth factor （VEGF） and superoxide dismutase （SOD） of acute myocardial infarction group were lower than those of tetramethylprazine group （ all P 〈 0.05 ）. VEGF, LDH were closely related with EF both in acute myocardial infarction group and tetramethylprazine group. Conclusion Tetramethylprazine have certain effect on protecting myocardium in rats with acute myocardial infarction by increasing the contents of VEGF in serum and the activity of SOD.
出处
《山东医药》
CAS
2013年第9期11-13,共3页
Shandong Medical Journal
基金
辽宁省高等学校优秀人才支持计划资助项目(LJQ2012072)
关键词
川穹嗪
急性心肌梗死
超声心动图
tetramethylprazine
acute myocardial infarction
echocardiography
