摘要
以苏丹红Ⅰ为模板分子,甲基丙烯酸为功能单体合成了苏丹红分子印迹聚合物,作为固相萃取吸附剂,用于食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的测定。样品经乙腈提取,所得提取液分子印迹固相萃取柱,然后用乙醇-正己烷(1+1)溶液作洗脱剂将苏丹红洗下,收集洗出液供高效液相色谱测定。结果表明:聚合物对印迹分子具有很好的亲和性和特异选择性。方法的线性范围为0.5～15 mg·L^(-1),检出限(3S/N)为4μg·kg^(-1)。以辣椒粉和辣椒酱为基体,在3个浓度水平下做加标回收试验,回收率在70.3%～85.5%之间,测定值的相对标准偏差(n=5)低于6.0%。
The molecularly imprinted polymer of Sudan red was synthesized with Sudan Ⅰ as template molecule and methacrylic acid as functional monomer, and it was used to extract Sudan Ⅰ-Ⅳ from food as adsorbent in solid phase extraction column. The sample was extracted with acetonitrile, and the extract obtained were purified on MIP-SPE column. Sudan Ⅰ-Ⅳadsorbed on the imprinted polymer were eluted with ethanol-n-hexane （1＋1） mixture, which were collected and used for HPLC determination. It was found that the imprinted polymer had good affinity and excellent selectivity for Sudan Ⅰ-Ⅳ. Linearity range for the determination was found between 0. 5-15 mg·L^-1 , and detection limit （3S/N） was 4μg·kg^-1. Using chili powder and chili sauce as matrixes, test for recovery was made at 3 concentration levels, values of recovery found were in the range of 70. 3%-85. 5% with RSD＇s （n=5） less than 6.0%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2013年第2期153-155,160,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
广东高校优秀青年创新人才培育项目(209030)
