摘要
综合运用硅胶、大孔吸附树脂、制备HPLC等柱色谱法分离纯化金鸡脚Phymatopteris hastata正丁醇部位的化学成分,从金鸡脚正丁醇提取部位中分离得到1个新化合物,采用NMR和质谱等波谱学方法鉴定新化合物的结构为(-)-表阿夫儿茶精-3,5-二-O-β-D-呋喃芹菜糖苷(1)。进一步通过测定化合物对胰岛素抵抗HepG2细胞葡萄糖消耗和骨骼肌细胞L6myc细胞葡萄糖转运子4(GLUT4)转位作用来探讨其对糖代谢的影响。抗糖尿病活性筛选结果表明,化合物1及其类似化合物-柚皮苷均能剂量依赖性提高胰岛素抵抗HepG2细胞的葡萄糖消耗量,并显著促进骨骼肌细胞L6mycGLUT4的转位作用。结果表明新化合物1和柚皮苷均具有潜在的体外抗糖尿病活性。
By repeated column chromatography, including silica gel, macroporous resin, and preparative HPLC, a new compound （ 1 ） was isolated and purified. On the basis of spectroscopic methods, the structure of 1 was elucidated as （ - ） -epiafzelechin-3, 5-di-O-β-D-apiofuranoside （1）. In the bioassay screening experiments, glucose consumption assays in IR HepG2 cells and colorimetric assay of surface GLUT4mye translocation were used to assess the effects on glucose metabolism of compound 1. Both compound 1 and its derivatives naringin could improve glucose consumption in IR HepG2 cells and enhance GLUT4 translocation in skeletal muscle cell L6myc in a dose-deoendent manner, indicating that these two comoouds showed potential anti-diabetic activities in vitro.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第6期831-834,共4页
China Journal of Chinese Materia Medica
基金
天津市科委重点基金项目(11JCZDJC20900)
关键词
假瘤蕨属
金鸡脚
黄烷醇苷
抗糖尿病活性
Phymatopteris
Phymatopteris hastata
flavanol glycoside
anti-diabetic activity
