摘要
目的定性定量地考察铜绿假单胞菌噬菌体PaP3在感染宿主菌PA3后对宿主转录组的全局性调控作用。方法利用高通量链特异性的RNA-seq对PaP3感染宿主菌PA3后5个时间点(5 min、10 min、20 min、30 min、80 min)的转录本进行深度测序,并以铜绿假单胞菌PAO1株及噬菌体PaP3基因组序列为参照,通过生物信息学对检测数据做定性定量的分析。结果以未感染噬菌体的细菌为对照,在铜绿假单胞菌PA3被噬菌体PaP3感染后的不同时间点共检测到5 536个差异表达基因,共归属于27条PseudoCAP功能注释,KEGG Pathway显著性富集分析发现差异表达基因共涉及45条代谢路径。此外,在5个样本中还预测出1 438个新转录本及1 329个新的候选sRNA,发现19种已注释的sRNA及91种新的候选sRNA出现差异表达。结论噬菌体PaP3可能通过抑制宿主菌PA3转录调节相关基因而对宿主的转录组进行全局性的调控,这为深入理解噬菌体与宿主相互作用的机制奠定了基础,也为探索噬菌体通过细菌与人体免疫系统的相互作用提供了多方面多层次的信息。
To investigate the global regulatory effect of P. aeruginosa phage PaP3 on the transcription of host PA3, the transcriptomes of P. aeruginosa at different time （5 min, 10 min, 20 min, 30 min, and 80 rain） after PaP3 infection were deeply sequenced via high-through RNA-seq technology. Based on P. aeruginosa PAO1 and R aeruginosa phage PaP3 genomic sequence, the sequencing data was analyzed quantitatively and qualitatively with bioinformatics approach. A total of 5 536 differential expression genes were identified, which were further classified into 27 PseudoCAP annotations or 45 metablic pathways. In addition, 1 438 novel transcripts and 1 329 candidates sRNA were identified. The expression of 19 known sRNA and 91 candidates sRNA were significantly changed. The result indicated that P. aeruginosa phage PaP3 probably globally regulates the transcriptome of the host PA3 by down regulating the expression of transcriptional regulator. Our study will not only be a basic for further understanding the relationship between bacteriophage and its host bacterium, but also provide multi-level information of bacteriophage use to influence human＇ s immune system through bacterium.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2013年第4期277-285,共9页
Immunological Journal
基金
国家自然科学基金面上项目(30770093)
重庆市自然科学基金重点项目(cstcjjB10018)
