摘要
为探明分布于中国四大海区的天然三疣梭子蟹群体脂肪酸组成状况及群体差异,并建立群体判定的指纹标记以应用于种质鉴定和资源保护,实验应用脂肪酸指纹标记结合多元分析方法在表型水平上研究中国四大海区中湛江、大连、连云港、东营、舟山和漳州海区6个天然群体秋冬季三疣梭子蟹的脂肪酸差异,并建立了群体判定的脂肪酸指纹标记。结果表明,四大海区6群体三疣梭子蟹脂肪酸存在显著差异(P<0.01),均含有27种脂肪酸。其中,反式油酸、棕榈酸、DHA、EPA和芥酸这5种脂肪酸的总含量在6群体中均高达82%以上。油酸、二十碳一烯酸、芥酸、ARA和EPA这5种脂肪酸作为脂肪酸指纹标记可以有效的对中国四大海区的6群体秋冬季雌性三疣梭子蟹进行种质鉴定,其综合判别准确率达88.46%。应用所含的27种脂肪酸进行聚类分析显示,四大海区6群体三疣梭子蟹系统聚类结果与地理距离不具有显著相关性,这种脂肪酸组成的群体差异可能与栖息地食物组成差异有关。本研究结果对于中国四大海区三疣梭子蟹的种质鉴定和原产地资源保护具有一定应用价值。
The swimming crab is widely distributed in the coastal waters of China, Japan and Korea. This species is one of the most common edible marine crab in the East Asia. To assess the fatty acid composition and variation of swimming crab populations and to develop a genetic resources identification technique based on fatty acids biomarkers, a multivariate analysis method was used to study fatty acid composition difference of swimming crab P. trituberculatus along China coast, the six geographical populations from China Sea （ Dalian, Dongying, Lianyungang, Zhangzhou, Zhoushan and Zhanjiang） in autum and winter. A set of fatty acids as fingerprint technique was developed for identifying different populations. The crabs from the six population all contained 27 kinds of fatty acids among which the total volume of five kinds of fatty acids C18： l n9t,C16： 0, C22： ln9, DHA and EPA was over 82%, and multivariate ANOVA revealed that there were significant differences among the six populations（ P 〈 0.01 ）. As fatty acid fingerprint markers, the five kinds of fatty acids C18 ： lngc, C20 ： 1, C22 ： ln9, ARA and EPA, were effective to identify the six- populations of crabs for the total discriminant accuracy amounted to 88.46%. The results of cluster analysis indicated that there were no significant correlations between geographical distance and Euclidean distance based on 27 kinds of fatty acids. The reason of population differences of fatty acids was probably related with feed composition in habitat of the swimming crabs.
出处
《水产学报》
CAS
CSCD
北大核心
2013年第2期192-200,共9页
Journal of Fisheries of China
基金
国家"十二五"科技支撑计划重大项目(2011BAD138B03)
国家自然科学基金项目(31072213)
江苏省海洋生物技术重点实验室开放课题(2006HS002)
关键词
三疣梭子蟹
地理群体
脂肪酸
气相色谱法
多元分析
指纹标记
Portunus trituberculatus
geographical population
fatty acid
gas chromatography
multivariate analysis
fingerprint technique
