摘要
目的观察舒郁宁心方对慢性应激抑郁模型大鼠行为学以及海马脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)及其主要受体酪氨酸激酶B(tyrosine kinase B,TrkB)表达的影响,探讨其抗抑郁机制。方法将60只成年SD大鼠随机分为正常对照组、模型组、西药组及中药大、中、小剂量组,每组10只。对大鼠进行21天的应激刺激建立慢性应激抑郁模型。除正常对照组外,其他5组大鼠在造模第22天开始灌胃,模型组灌胃生理盐水,中药大、中、小剂量组分别给予25.0、7.5、2.5g/kg舒郁宁心方灌胃;西药组给予盐酸氟西汀混悬液(12mg/kg)灌胃;均连续给药3周。分别于0(造模前)、3周(造模成功后)、6周(给药结束后)测定每只大鼠体重;敞箱实验检测大鼠行为学;强迫游泳实验记录大鼠5min内不动时间。实验结束后脑区取材,测定大鼠脑组织中BDNF及TrkB表达水平。结果与正常对照组比较,模型组大鼠体重增长缓慢,行为学指标下降,强迫游泳不动时间延长,海马BDNF及TrkB表达减弱,差异均有统计学意义(P<0.05,P<0.01)。给药结束后,中药大剂量组和西药组大鼠体重增加,大鼠行为学改善,强迫游泳不动时间缩短,海马BDNF、TrkB表达增强,与模型组比较,差异均有统计学意义(P<0.05,P<0.01)。结论舒郁宁心方可能通过改善脑组织BDNF和TrkB的表达水平发挥抗抑郁作用。
Objective To observe the effects of Shuyu Ningxin Recipe （SNR） on the praxiology and the expressions of hippocampal brain-derived neurotrophic factor （BDNF） and its receptor tyrosine kinase B （TrkB） of model rats with chronic stress-induced depression, thus exploring its anti-depression mechanisms. Methods Sixty adult SD rats were randomly divided into 6 groups, i.e., the normal control group, the model group, the fluoxetine group, the high dose SNR group, the medium dose SNR group, and the low dose SNR group, 10 in each group. All rats were subjected to establish chronic stress-induced depression model for 21 consecutive days. Except those in the normal control group, rats in the rest groups received gastrogavage from the 22nd day. Mice in the model group were administered with normal saline by gastrogavage. SNR at 25. 0, 7. 5, and 2. 5 g/kg was respectively administered to rats in the high dose SNR group, the medium dose SNR group, and the low dose SNR group by gastrogavage. Ruoxetine suspension （12 mg/kg） was given to rats in the fluoxetine group by gastro- gavage. All medication lasted for 3 successive weeks. The weight, open-field test, and the immobility time in forced swimming test were determined before modeling, 3 weeks （after successful modeling）, and 6 weeks （by the end of medication）. The expressions of hippocampal BDNF and TrkB were measured after the brain tissues were drawn by the end of the experiment. Results Compared with the normal control group, the body weight grew slowly, the behavior index decreased, the immobility time in forced swimming test was prolonged, and the expres- sions of BDNF and TrkB were weaken in the model group （P 〈0. 05, P 〈0. 01 ）. The body weight increased, the be- havior was improved, the immobility time in forced swimming test was shortened, and the expressions of BDNF and TrkB were enhanced in the high dose SNR group and the fluoxetine group by the and of medication, showing statistical difference when compared with the model group （P
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第3期370-375,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
北京科委课题首都特色临床医学课题(No.Z090507017709030)
军队十二五科研攻关项目(CWS.12J129)
关键词
抑郁
脑源性神经营养因子
酪氨酸激酶B
舒郁宁心方
depression
brain-derived neurotrophic factor
tyrosine kinase B
Shuyu Ningxin Recipe
