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母乳中甲硝唑药物代谢动力学的毛细管电泳研究 被引量:2

Study on the Pharmacokinetics of Metronidazole in Human Milk by Capillary Electrophoresis
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摘要 用毛细管电泳测定了甲硝唑在母乳中的含量 ,研究了其药代动力学。以液 -液萃取作为样品预处理手段 ,萃取回收率在90%~101 %范围内。以替硝唑作为内标 ,采用场放大进样。该法在甲硝唑质量浓度为0.40~25.67mg/L时 ,甲硝唑质量浓度和甲硝唑与替硝唑的峰面积之比呈线性 ,线性相关系数 (r)大于0.999,最低检测量为0.40mg/L ,检出限 (S/N=3)为0.15mg/L ,相对峰面积的相对标准偏差为1.5 %。 The determination of metronidazole in human milk by capillary electrophoresis is reported. The method can also be employed for pharmacokinetic studies. After single vein injection of 1.25 g metro_nidazole, human milk samples were collected within 24 hours. With chloroform as the extraction solvent, the extraction recovery was found to be in the range from 90% to 101%. To decrease the detection limit, sample _ stacking injection was employed. Samples with tinidazole as an internal standard in 40 mmol/L Tris-phosphate buffer(pH 2.0) were analyzed. The linearity for metronidazole against the internal standard was given to be in the range of 0.40~25.67 mg/L, and the correlation coefficient r was greater than 0.999. The limit for quantification was 0.40 mg/L, whereas the detection limit(S/N=3) was 0.15 mg/L. The relative standard deviation(RSD) of repeatability for corrected metronidazole peak area against the internal standard was 1.5%. The method was used to get pharmacokinetic parameters with the aid of the computer program MCPKP.
出处 《分析测试学报》 CAS CSCD 2000年第4期5-7,共3页 Journal of Instrumental Analysis
基金 国家自然科学基金!资助项目 (29875033)
关键词 毛细管电泳 母乳 甲硝唑 药代动力学 Capillary electrophoresis Human milk Pharmacokinetics Metronidazole
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  • 1戴自英,实用抗菌药物学,1992年,210,96,287页 被引量:1

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