摘要
目的观察大豆异黄酮对急性低压缺氧大鼠学习记忆能力影响,初步探究其作用机制。方法低压氧舱建立大鼠缺氧模型,分别给予高、中、低剂量大豆异黄酮,Morris水迷宫观察大鼠学习记忆能力,生化法检测大鼠海马匀浆中超氧化物歧化酶(SOD)及丙二醛(MDA)水平,免疫组化法观察海马细胞Bcl-2与caspase-3蛋白表达。结果模型组大鼠海马SOD活力与MDA含量分别为(76.04±16.57)U/mL、(0.87±0.15)nmol/mL,Bcl-2、caspase-3蛋白表达分别为(0.84±0.15)、(0.64±0.10),与对照组比较,大鼠学习记忆能力明显降低,海马SOD水平与Bcl-2表达量均降低(P<0.01),MDA水平与caspase-3表达量均增加(P<0.01);高剂量大豆异黄酮组大鼠海马SOD与MDA分别为(109.43±23.73)U/mL、(0.75±0.11)nmol/mL,Bcl-2、caspase-3分别为(0.99±0.17)、(0.49±0.08),与模型组比较,SOD水平与Bcl-2表达量有增加趋势,MDA含量与caspase-3表达量有降低趋势。结论大豆异黄酮可增强低压缺氧大鼠学习记忆能力,其机制与增加缺氧大鼠海马组织抗氧化能力,调节凋亡通路蛋白表达有关。
Objective To examine the effect of soy isoflavones on learning and memory abilities of rats after acute hypobaric hypoxia, and to explore the mechanism of the effects. Methods The hypoxic model was established with low pressure chamber. The high, moderate, and low dose of soy isoflavones were administered to the rats exposed to hypoxia and learning and the memory abilities of the rats were detected with Morris water maze. The contents of superoxidase dis- mutase（SOD） and malondialdehye （MDA） in hippocampal homogenate of the rats were determined with bilochemical assay. The protein expressions of Bcl-2 and caspase-3 in hippocapal cells were detected with immunohistochemical method. Results Compared to the control group, the learning and memory abilities of the model group was significantly weakened;hippocampal SOD level and Bcl-2 expression were significantly lower（76.04 ±16. 57 vs 117.03 ±20. 10 U/mL and 0. 84± 0. 15 vs 1.76 ±0. 13, P 〈 0. 01 for all）, whereas the MI）A levels and caspase-3 expression were significantly higher（0. 87± 0. 15 vs 0. 70 ±0. 11 nmoVmL and 0. 64±0. 10 vs 0. 64±0. 09,P〈0. 01 for all）. Compared to those of the model group,the SOD level（ 109.43 ± 23.73 μ/mL） and the expression of Bcl-2 （0. 99 ± 0. 17 ） increased, and MDA content（0. 75±0. 11 nmol/L） and the expression of caspase-3（0. 49±0. 08） decreased in the rats of high dose soybean isofla- vones treatment group. Coneluslon Soy isoflavones enhance study ability of the rats with hypobaric hypoxia and the mecha- nism of the effect is related to the increase of antloxidant capacity of hippocampus and the regulation of apoptotic pathway.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第3期373-375,共3页
Chinese Journal of Public Health
基金
吉林市科技发展计划项目(吉市科社字2008051)
吉林省教育厅"十一五"科学技术研究项目(吉教科合字2010-255)
关键词
大豆异黄酮
低压缺氧
学习记忆
机制
soy isoflavones
hypobaric hypoxia
learning and memory
mechanism
