摘要
目的探讨细胞因子在肝吸虫发病机制中的作用和中药黄芪保护肝组织的机制。方法健康成年Wistar大鼠32只随机分为对照组、模型组、阿苯达唑（ALB）治疗组和阿苯达唑联合黄芪（ALB＋MR）治疗组，建立肝吸虫病大鼠动物模型。ALB治疗剂量50mg／kg／d／只，连用5天；MR腹腔注射800mg／ks／d／只，连用30天。感染14周后处死大鼠，光镜观察大鼠肝组织病理改变，ELISA法检测大鼠血清细胞因子IL-2和IL-4水平。结果对照组大鼠肝组织未见异常，模型组大鼠肝组织病理改变显著，阿苯达唑和黄芪治疗后病理改变明显减轻。与对照组比较，模型组大鼠血清IL-2水平下降（F=18．3，P=0．0002），IL-4水平升高（F=10．44，P=0．0034）。治疗后，ALB组和ALB＋MR组大鼠血清IL-2水平升高（F=8．69，P=0．0068；F=5．5，P=0．0273），IL-4水平下降（F=9．69，P=0．0046；F=7．61，P=0．0107）阿苯达唑联合黄芪治疗效果更好。结论细胞因子IL-2和IL4参与肝吸虫引起的肝组织损伤，黄芪对肝组织具有保护作用，其机制可能与调节紊乱的细胞因子有关。
Objective To investigate the changes of cytokines in rats with Clonorchiasis and the protective effects of Milkvetch Root on liver tissue. Methods Adult 32 male rats were randomly divided into four groups ： control group, model group, Albendazole （ALB） group and Albendazole combined with Milkvetch Root （ ALB ＋ MR） group （8 rats / group）. The experimental rats were infected with 50 metacercariae of C. siuensis or equal physiological saline through a garage needle. The ALB group and ALB ＋ MR group received ALB （50mg/kg/d） orally for 5days, or with MR intraperitoneally （800 mg/kg/d） for 30 days. All rats were saeririced after 14 weeks of infection. Histopathological examination of rats＇ livers was performed. The levels of IL- 2 and IL-4 in the serum of the rats were detected by ELISA assays. Results The vacuolization of hepatocytes and infiltration of white cells were observed in the model group, but did not observed in control group under light microscope. The injury of liver tissue of rats in ALB ＋ MR group was milder than ALB group. The level of IL-4 in serum of rats in model group increased significantly（ F = 10. 44,P = 0. 003 4） , but the level of IL-2 de- creased significantly compared with control group（ F = 18.3 ,P = 0. 000 2 ）. The level of IL-4 in serum of rats in ALB group and in ALB ＋ MR group decreased significantly （ F = 9.69, P = 0. 0046 ; F = 7.61, P = 0. 010 7 ）, while the level of IL-2 of rats in ALB group and in ALB ＋ MR group increased significantly （ F = 8.69, P = 0. 006 8 ; F = 5.5, P = 0. 027 3 ）. Conclusion Our results suggested that IL-2 and IL-4 were involved in the pathogenesis of clonorchis sinensis. Milkvetch Root could provide protection to the liver tissue against Clonorchis sinensis, which might associate with the regulation of disturbed cytokines.
出处
《国际免疫学杂志》
CAS
2013年第2期138-141,共4页
International Journal of Immunology
基金
黑龙江省教育厅面上基金资助项目，黑龙江省自然基金资助项目
关键词
肝吸虫
细胞因子
黄芪
阿苯达唑
Clonorchis sinensis
Cytokine
Milkvetch Root
Albendazole
