摘要
目的观察中药慈菇消脂丸对大鼠非酒精性脂肪性肝病的防治作用。方法建立非酒精性脂肪性肝病大鼠模型,随机分为正常对照组、模型对照组、慈菇消脂丸高剂量组、慈菇消脂丸低剂量组、阳性对照组,观察肝组织病理形态学的变化。免疫组化技术检测内皮型一氧化氮合酶(eNOS)及诱导型一氧化氮合酶(iNOS)在肝脏的表达。结果模型组大鼠肝组织出现脂肪变性及不同程度的炎性细胞浸润,镜下可见肝细胞内大量脂滴。与正常组相比较,模型组大鼠肝组织内iNOS蛋白表达显著提高,eNOS蛋白表达降低(P<0.01);与模型组相比较,中药治疗组大鼠肝组织内iNOS蛋白表达显著降低,eNOS蛋白表达提高;与阳性药物对照组相比较,中药慈菇消脂丸高剂量组作用显著(P<0.05)。结论慈菇消脂丸对大鼠非酒精性脂肪性肝病具有明显的防治作用,其作用机制与改善肝组织脂肪变性程度、抑制脂质过氧化、抗炎有关。
AIM To observe preventive effects of Cigu Xiaozhi Pills on non-alcoholic fatty liver disease of rats.METHODS Non-alcoholic fatty liver disease rats were induced by feeding high-cholesterol diet and were divided randomly into five groups,normal group,model group,high,low dosage of the treatment groups and positive drug group.Histopathological changes in the liver were observed.Expressions of endothelial cell nitric oxide synthase（eNOS） and inducible nitric oxide synthase（iNOS） protein were determined by immunohistochemical technique.RESULTS In model group,extensive adipose degeneration and inflammatory cell infiltration were found in the liver.Compared with the normal group,the increase in expression of iNOS protein and the decrease in expression of eNOS protein were obvious in the model group（P ＆lt; 0.01）.Compared with the model group,the expression of iNOS protein decreased and the expression of eNOS protein increased in the treatment groups（P ＆lt; 0.01）.The effect of high dosage group was better than that of the positive drug group（P ＆lt; 0.05）.CONCLUSION Cigu Xiaozhi Pills can effectively treat and prevent experimental non-alcoholic fatty liver disease in rats,and its mechanism may be associated with ameliorating hepatocellular steatosis,removing the free radicals and enhancing the capability of anti-oxidation and anti-inflammatory.
出处
《中成药》
CAS
CSCD
北大核心
2013年第3期431-435,共5页
Chinese Traditional Patent Medicine
基金
兰州市科技局资助项目(05-1-31)
