摘要
目的：探讨位于脂氧酶12（LOXl2）基因编码区Arg261Gln单核苷酸多态与胃癌发病风险的关系。方法：用聚合酶链反应一限制性片段长度多态性分析（PCR—RFLP）方法检测148例胃癌患者和148例无肿瘤正常对照人群的LOXl2的基因型，并以Logistic回归模型计算各基因型与胃癌发病风险的关系。结果：LOXl2Arg261Gln等位基因频率在胃癌组中（0．544）高于正常组（0．443）。与Arg／Arg基因型携带者相比，Gln／Gln基因型携带者发生胃癌的风险增加（OR=2．26，95％CI=1．15。4．46，P=0．018），而杂合基因型Arg／Gln不增加胃癌发病风险（OR=1．37，95％CI=0．77～2．44，P=0．284）。结论：LOXl2编码区Arg261Gln遗传变异可能是胃癌发病的重要遗传易感因素。
Objective： To investigate the association ofArg261Gln polymorphism in the coding region of 12-1ipoxygenase （LOX12） gene with the risk of developing stomach cancer. Methods： The LOX12 genotypes in 148 patients with gastric cancer and 148 cancer-free controls were determined by using PCR-restriction fragment length polymorphism （PCR-RFLP）. The association between the genotypes and risk of developing gastric cancer was estimated by logistic regression model Results： The allele frequency for LOX12 Arg261Gln in gastric cancer patients （0.544） was higher than that of the normal controls （0.443）. Compared with the Arg/Arg genotype carriers, the risk of developing gastric cancer was increased in Gln/Gln genotype carriers （OR=2.26, 95%CI=1.15-4.46, P=0.018）, while those with the heterozygous genotype Arg/Gln showed no increased risk of developing gastric cancer （OR=1.37,95%CI=0.77-2.44, P=0.284）. Conclusion： The inherited polymorphisms ofArg261Gln in LOX12 gene encoding region may confer genetic susceptibility to development of stomach cancer.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第2期170-173,共4页
China Journal of General Surgery
基金
河北省卫生厅科技计划资助项目(20090624)
