摘要
将黏膜乳杆菌lm4208进行活菌培养和灭活处理,在不同初始菌体浓度、不同pH值条件下与玉米赤霉烯酮进行作用,采用HPLC方法测定培养液上清ZEN含量,确定黏膜乳杆菌lm4208对ZEN的清除率及各因素的影响。结果表明,黏膜乳杆菌lm4208对ZEN毒素具有较高的清除能力,清除率为51%～60%,灭活菌可显著提高对ZEN的清除效果。无论活菌还是灭活菌,初始菌体浓度对其清除效果有显著影响,pH值对清除ZEN能力无显著影响。本试验结果为进一步研究该菌株在实际生产中清除玉米赤霉烯酮的应用奠定基础。
In this study,the ability of L. mucosae lm4208 to remove zearalenone was studied at dif- ferent conditions in liquid . The viable or inactivated cells of L. mucosae lm4208 were cultured with zearalenone at different initial ceil concentration and different pH. The content of zearalenone in the culture supernatant was measured by HPLC method. The results showed that L. mucosae lm4208 has the high ability to remove zearalenone,and its removal ability was signifi- cantly increased when the bacteria was inactivated,and the initial cell concentration of the viable or inactivated L. mucosae lm4208 significantly influenced the removal ability, but the pH had no significant effect on the removal ability. The results layed the foundation for further study on the application of L. mucosae to the removal of zearalenone in practice.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第3期458-461,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31072178)
辽宁省博士启动基金资助项目(20111086)
中国博士后基金资助项目(2012M511772)
