摘要
为探讨肿瘤坏死因子 α(TNF- α)对 K5 6 2 细胞的细胞毒作用 ,应用原位末端标记法、细胞形态学观察、DNA琼脂糖凝胶电泳检测分析 K5 6 2 ,经 TNF- α(5 0 ng/ml、1 0 0 ng/ml、1 0 0 0 ng/ml)诱导的 K5 6 2 。结果显示 ,K5 6 2 细胞自然凋亡率 (3.5 0± 0 .97) % ,TNF- α(5 0 ng/m l、1 0 0 ng/ml)诱导的 K5 6 2 细胞凋亡率分别是 (2 1 .35± 4.46 ) %、(2 7.38±4.91 ) % ,差异有显著性 (P<0 .0 1 ) ;TNF- α(1 0 0 0 ng/ml)诱导 K5 6 2 细胞坏死。说明小剂量 TNF- α诱导 K5 6 2 细胞凋亡 ,大剂量 TNF- α诱导 K5 6 2
To explore whether TNF α can induce K 562 . cells' apoptosis or necrosis,TDT end labelling techniques,cell morphology and DNA agarose gel electrophoresis were used to detect K 562 cells, K 562 cells induced by a different dose of TNF α.The results:K 562 cells natural apoptotic percentage was( 3.50 ± 0.97 )%, The percentage of K 562 cells'apoptosis induced by TNF α(50 ng/ml,100 ng/ml)was(21.35±4.46)%, (27.38±4.91)%,respectively,The difference is significant(P<0.01).TNF α(1 000 ng/ml)induced K 562 cells'necrosis.These indicated that K 562 cells were induced to be apoptosis in a small dose of TNF α,induced to be necrosis in large dose of TNF α
出处
《医学新知》
CAS
2000年第3期126-127,共2页
New Medicine
