摘要
目的探讨特定序列人工合成的寡核苷酸(Oligodeoxynucleotide,ODN)MT01对人骨髓间充质干细胞(Human bone marrow mesenchymal stem cells,hBMSCs)增殖及成骨分化的影响。方法采用Ficoll梯度密度离心法分离培养hBMSCs并进行鉴定。以1μg/mL的ODN MT01处理hBMSCs,细胞计数试剂盒检测细胞增殖情况,碱性磷酸酶试剂盒检测成骨分化情况。结果经1μg/mL的ODN MT01处理的hBMSCs体外培养,第3、4、5、6、7天hBMSCs增殖明显;成骨诱导的第4、14、21天,碱性磷酸酶表达明显增加。结论特定浓度人工合成ODN MT01能够在体外促进hBMSCs的扩增及成骨分化。
Objective To investigate the influences of specific synthetic oligodeoxynucleotides （ODN） on the proliferation and osteogenie differentiation of hBMSCs. Methods The hBMSCs were isolated via Ficoll density gradient centrifugation and then identified after amplification. Then the hBMSCs were cultured by synthetic ODN MT01 with a eentrifugation of 1 μg/mL. Proliferation of hBMSCs were measured using cell counting kit, and the influences on osteogenic differentiation were detected by alkaline phosphatase kit. Results The growth of hBMSCs was promoted by ODN MT01 with a eentrifugation of 1μg/ml on the 3, 4, 5, 6, 7 day, and the ATP activity of hBMSCs was also promoted on the 4, 14, 21 day after induced. Conclusion Certain concentration of synthetic ODN MTO1 can promote the growth and osteogenic differentiation of hBMSCs in vitro.
出处
《组织工程与重建外科杂志》
2013年第1期1-4,共4页
Journal of Tissue Engineering and Reconstructive Surgery
基金
佳木斯大学重大培育基金项目(SZP2001-001)
关键词
骨髓间充质干细胞
寡核苷酸
增殖
成骨分化
Bone marrow mesenchymal stem cells
Oligodeoxynucleotide
Proliferation
Osteogenic differentiation
