摘要
分离纯化获得氮胁迫条件下稻瘟病菌CH-63分泌产生的致病蛋白Mg-p1,大小约为58 KD。该蛋白接种感病品种蒙古稻,接种处叶片出现过敏性坏死反应。电镜检测接种该蛋白后叶片细胞超微结构变化,发现与对照相比致病蛋白处理后细胞壁加厚,形成空腔,叶绿体膜消失,淀粉粒数量减少,叶绿体发生崩解。构建抗、感水稻品种C101A51和蒙古稻的悬浮细胞系,对致病蛋白Mg-p1接种后苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)活性变化进行检测。结果表明Mg-p1接种蒙古稻后PAL酶活增加强于完全培养条件下产生的分泌蛋白,而接种抗病品种C101A51后PAL活性变化不明显。在检测POD活性变化过程中,发现无论是感病或抗病品种,其互作过程中POD与对照相比无明显变化,这一现象说明了在水稻受到外源物侵染后,其POD活性均会发生变化,无明显专一性,PAL则针对感病品种具有稻瘟病菌致病特异性,而抗病品种也无明显专一性。通过上述研究对深入了解稻瘟病菌与水稻之间的相互识别,信号传导和应答过程,阐明水稻对稻瘟病菌的抗性机制、水稻与稻瘟病菌互作的分子机理具有重要意义。
The pathogenic secreted protein Mg-pl of rice blast CH-63 was extracted under nitrogen starvation, and the size was about 58KD. The Mongolia rice plant was inoculated by Mg-pl protein, and the hypersensitive response appeared in the rice leaves inoculated. The cellu- lar ultrastructuro changes of rice leaves inoculated with this protein were detected by electron microscope, indicating the cell wall was thick- ened to form cavity, the chloroplast envelope disappeared, the starch granules degraded and the chloroplast collapsed compared with the con- trol. The cell suspension lines of rice cl01A51and Mongolia rice were built. Inoculated by Mg-pl protein, the phenylalnine ammonialyase （PAL） and peroxidase （POD） activities were tested. The result showed that the PAL enzyme of Mongolia rice activity inoculated by Mg-pl protein was stronger than that of the secreted protclnin complete nutrition medium. The PAL enzyme of rice cl01ASl activity inoculated by Mg-pl protein was the same as that in the control. The peroxidase （POD） enzyme activities tested indicated that there was no significant difference in the POD enzyme activities of susceptibility or resistant variety rice compared than the control in interaction process. The result showed that the change of POD enzyme activities occurred when the rice was inoculated by exogenous substance with no obvious specificity. The PAL enzyme activities were specific to Magnaporthe grisea pathogenicity in the susceptible variety of rice inoculated by Mg-pl protein, but there was no obvious specificity in the resistant variety rice. The rice resistance mechanism and the molecular mechanism of rice blast with rice interaction were revealed through the study.
出处
《西南农业学报》
CSCD
北大核心
2013年第1期148-154,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31060021)
云南省自然科学基金项目(2006C0062M,2008ZC100M)
关键词
氮胁迫
稻瘟病菌
致病性分泌蛋白
苯丙氨酸解氨酶
过氧化物酶
超微结构
Nitrogen starvation
Rice blast
Pathogenicity secretedprotein
Phenylalanine ammonia-lyase
Peroxidase
Activities
