摘要
目的:制备负载碱性成纤维细胞生长因子(bFGF)的壳聚糖微球,并测其体外释药特性。方法:采用乳化交联法制备壳聚糖空白微球,再进行bFGF吸附,制成负载bFGF的壳聚糖微球,并对微球进行扫描电镜观察。ELISA法测定其载药量与包封率,以及bFGF的体外释放情况。并测定壳聚糖空白微球的吸水膨胀率。结果:负载bFGF的壳聚糖微球直径约4μm,载药量为1.06μg/mg,包封率为84.8%。体外释放实验初期释放bFGF速度较快,随后逐渐趋于平缓。壳聚糖空白微球的吸水膨胀率为650%。结论:采用乳化交联法制备包裹bFGF的壳聚糖缓释微球,简单易行,成球性好,得率高,具有良好的缓释效果。
Objective：To prepare basic fibroblast growth factor （bFGF） loaded chitosan microspheres, and to an- alyze the property of controlled release of bFGF in vitro. Methods： Firstly, chitosan blank microspheres were pre- pared with emulsification cross - linking method, then bFGF - loaded chitosan microspheres were prepared by the ch- itosan blank microspheres absorbing bFGF. The microspheres were observed by scanning electron microscopy, and the drug loading, encapsulation efficiency and in vitro controlled release of the bFGF - loaded chitosan microspheres were measured by ELISA. Furthermore, the water absorption expansion rate of microspheres were measured. Results： The mean diameter of the bFGF - loaded chitosan microspheres was about 4 μm, and the drug loading and encapsulation efficiency of the bFGF - loaded chitosan microspheres were 1.06 μg/mg and 84.8% , respectively. The micro- spheres released quickly at initial 12 hours, and then slowed gradually in vitro. The absorbing water expansion rate of the blank microspheres was 650%. Conclusion： Chitosan microspheres encapsulating bFGF prepared by emulsifica- tion cross -linking method exhibit high yield and good controlled -release effect.
出处
《中国卫生检验杂志》
北大核心
2013年第2期265-267,共3页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金面上项目(81071467)
浙江省医学重点学科群项目(XKQ-010-001)生物医药重点学科群
浙江省医药卫生科技计划项目(2010KYB001
2011KYA001)
关键词
碱性成纤维细胞生长因子
壳聚糖
缓释微球
体外释放
Basic fibroblast growth factor
Chitosan
Sustained- release microspheres
Release in vitro
