摘要
采用水蒸气蒸馏法,馏出液于pH 3.0、室温条件下与2,4-二硝基苯肼进行衍生化反应4 h后,其衍生化产物用高效液相色谱法,二极管阵列检测器,C18色谱柱,以乙腈:水=60:40(体积比)为流动相,流速1 mL/min,于波长350 nm处测定甲醛衍生物含量。本文对食用菌中甲醛的提取方式、衍生化反应的时间、衍生化试剂的浓度、衍生反应的温度、pH、检测波长的选择、流动相的选择分别进行了讨论,得到方法检出限为0.02 mg/kg,回收率大于85%,相对标准偏差≤1.29%(n=6),该方法选择性强,操作简便,用于实际样品的测定,结果满意。
Determination of formaldehyde by HPLC in edible fungi was reported in this paper. The formaldehyde in the sample was reacted with the derivation reagent, 2,4-dinitrophenylhydrazine, in an acidic medium to form 2,4-dinitrophenylhydrazone, which after an appropriate pretreatment was separated on the C 18 column, and detected at 350 nm on DAD. Qualitative examination was made using the retention time and quantitative estimation was made by measuring the peak area. Detection limit of the method was found to be 0.02 mg/kg, and its recoveries were greater than 85 %. An average value of RSD of 1.29 % （n=6） was obtained. The method had been applied for the determination of the trace amount of formaldehyde in samples, and satisfactory results were obtained.
出处
《食品研究与开发》
CAS
北大核心
2013年第2期65-67,共3页
Food Research and Development
关键词
高效液相色谱法
食用菌
甲醛
high performance liqiud chromatography
edible fungi
formaldehyde
