摘要
以番茄灰霉菌（Botrytis cinerea）、菊芋菌核菌（Jerusalem artichoke Sclerotium）、油菜菌核菌（Sclerotinia sclerotiorum）、辣椒疫霉菌（Phytophthora capsici）、蚕豆根腐菌（Fusarium solani）及豌豆根腐菌（Aphanomyces euteiches Dreehsler）等6种植物病原真菌为供试菌株,采用滤纸片法对植物病原菌提取液的抑菌活性进行了测定。为了获得对这几种植物病原菌具有较高抑制作用的嗜盐菌菌株,对供试菌株进行了液体发酵及发酵液的活性筛选。经滤纸片法测定,青海盐湖嗜盐菌菌株对辣椒疫霉菌具有较强的拮抗作用,并用最小抑制浓度法（MIC）测定了嗜盐菌提取物对辣椒疫霉菌的最小抑菌浓度范围为9~12 mg/mL。
Six plant pathogenic fungi including Botrytis cinerea, Jerusalem artichoke Sclerotium, Sclerotinia sclerotiorum, Phytophthora ctrpsici, Fusarium solani, and Aphanomyces euteiches Dreehsler were used to determine the inhibitory activity of fermentation broth of halophilic bacteria against plant pathogenic fungi by paper disc diffusion method. In order to find halophilic strain with higher inhibitory activity against the plant pathogenic fungi, the tested strains were fermented to screen. The halophilic strains were with certain antagonistic activity against the growth of P. capsici by the disc diffusion assay. And the minimum inhibitory concentration （MIC） of fermentation broth of halophilic strains against P. capsici ranged from 9 to 12 mg/mL.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第1期79-81,88,共4页
Guangdong Agricultural Sciences
基金
青海省科技厅应用基础研究项目(2011-Z-727)
青海省农林科学院创新基金(2011-NKY-01)
关键词
嗜盐菌
次级代谢产物
滤纸片法
抑菌活性
inhibitory activity
plant pathogenic fungi
halophilic bacteria
paper disc diffusion method
