摘要
目的观察胰岛素抵抗（IR）大鼠血清淀粉样蛋白A（SAA）含量、主动脉抵抗素样分子α（RELMα）表达情况以及游泳运动干预后上述指标的变化。方法6～8周龄体重160—200gWistar雄性大鼠30只，采用随机数字表法分为正常对照组（A组）和IR组（B组）、运动组（C组）各10只，正常对照组给予普通饲料喂养，后两组大鼠均给予高脂高糖饮食喂养，8周后经计算稳态模型IR指数（HOMA-IR）确认IR大鼠造模成功后，继续给予原饮食喂养8周，C组同时进行8周游泳运动。16周末测量各组大鼠体重、空腹血糖（FBG）、空腹胰岛素（FINS）、甘油三酯（TG）、总胆固醇（Tc）、高密度脂蛋白胆固醇（HDL—C）及低密度脂蛋白胆固醇（LDL-C）、RELMα、血清淀粉样蛋白A（SAA）、HOMA-IR；苏木精一伊红（HE）染色观察各组主动脉形态学变化。各组间计量资料比较采用单因素方差分析。结果与正常对照组相比，IR组大鼠体重、FBG、FINS、HOMA—IR、TC、TG、LDL—C、SAA、RELM（x水平均明显升高，差异均有统计学意义（t=-20．666～-3．681，均P〈0．05），HDL—C明显降低，泡沫细胞数明显增多，差异有统计学意义（t=8．950、-20．596，P〈0．05），IR组主动脉内皮排列紊乱、内膜增厚；运动组干预后SAA、RELMα、体重、FBG、FINS、HOMA．IR、TC、TG、LDL—C均较IR组明显下降，差异均有统计学意义（t=2．524～8．640，均P〈0．05），相应泡沫细胞聚集程度减轻（t=11．520，P〈0．05）。结论RELMα、SAA与IR、主动脉炎症关系密切；运动能降低SAA、RELMα水平及泡沫细胞聚集程度，对改善IR、减轻IR大鼠主动脉炎症反应具有重要意义。
Objective To investigate the effects of exercises on expressions of serum amyloid A protein （ SAA ） and resistin-like molecules α （RELMα） in aorta of insulin-resistant （ IR ） rats induced by diets. Method Taking the completely randomized method, a total of 30 Wistar rats were divided into 3 groups：normal control group（ n = 10） fed with normal diet, IR and exercises groups with high sugar and high fat diet（ 10 rats in each group）. Eight weeks later, the index of insulin resistance was assessed to confirm that models were successfully made. The three groups were continually fed with the same diet as before, while the exercise group was administered with swimming training for eight weeks in addition. At the end of sixteen weeks experiment, Rats＇ blood were sampled for measuring the levels of triglyceride （TG） ,total cholesterol （ TC）, high-density lipoprotein cholesterol （ HDL-C ）, low-density lipoprotein cholesterol （ LDL-C ）, glucose （FBG） ,SAA, aorta RELMoL mRNA and insulin （FINS） and the body weight （BW） were measured. The homeostasis model assessment index of IR（HOMA-IR） were calculated. Haematoxylin and eosin stain method was applied to observe morphology characteristics of aorta in groups. Difference of measurement data was compared with single factor analysis of variance. Results The levels of BW, FBG, FINS, HOMA-IR, TC, TG,LDL-C, SAA, RELMα mRNA in IR group were higher than those in control group （t = -20. 666 -3. 681 ,all P 〈0. 05） ,and the number of foam cell increased well （t = - 20. 596, P 〈0.05） ,while the levels of HDL-C were lower （ t = 8. 950, P 〈 0. 05 ）. The endothelial in the IR group were in disorder, and neointimal hyperplasia was found. After the intervention of exercise, the levels of SAA, RELMot mRNA, BW,FBG, FINS, HOMA-IR, TC, TG, LDL-C excercises group were decreased than those in IR group （ t = 2. 524 - 8. 640, P 〈 0. 05 ）, and the number of foam cell decreased well （ t = 11. 520, P 〈 0. 05 ）, while
出处
《中华糖尿病杂志》
CAS
CSCD
2013年第2期102-107,共6页
CHINESE JOURNAL OF DIABETES MELLITUS
