摘要
利用石蜡切片法对欧洲七叶树体细胞胚胎发生进行细胞组织学观察,结果表明:胚性细胞具有细胞小、大小均一、胞质浓、细胞核大、核仁明显等特点,且分裂速度快:而非胚性细胞体积较大、核小、细胞质稀薄、细胞之间间隙较多,不易成团。体细胞胚发生主要起源于表层的单细胞,依次经过2-细胞原胚、多细胞原胚、球形胚、心形胚、鱼雷形胚和子叶胚等与合子胚发育类似的过程,再生植株。欧洲七叶树离体本培养产生的体细胞胚具备发育完善的V型维管束、茎尖分生组织以及根尖分生组织,具有明显的两极性和相对的独立性。
The origin and development of somatic embryos of Aesculus hippocastanum were ob- served by a paraffin method. The results showed that the embryonic cells had such characteristics as small and even size,dense kytoplasm,large karyon and obvious nucleolus as well as rapid division rate, while the non-embryonic cells had large size, small karyon, thin kytoplasm and much intercellular space and were not easy to agglomerate. The somatic embryogenesis mainly originated from surface unicells, and the regenerated plantlets were developed in turn as 2-cell proembryo, multicelluar proem- bryo,globular embryo,heart-shape embryo,torpedo-shape embryo and cotyledon embryo, being simi- lar to a development process of zygotic embryo. The somatic embryos produced by in vitro culture had full-grown V-shaped vascular bundles,stem apex meristem and root tip meristem,possessing a distinct bipolarity and relative independence.
出处
《上海农业学报》
CSCD
北大核心
2013年第1期24-28,共5页
Acta Agriculturae Shanghai
基金
上海市科技兴农重点攻关项目"优良栎类树种单株选优和扩繁技术研究"[沪农科攻字(2009)第9-5号]资助
关键词
欧洲七叶树
体细胞胚
Ⅴ型维管束
两极性
Aesculus hippocastanum
Somatic embryo
V-shaped vascular bundle
Bipolarity
