摘要
目的 探讨肝癌患者转化生长因子 β1(TGF β1)及其受体的表达情况和意义。方法 酶联免疫吸附法检测 2 7例行胆囊切除术者 (对照组 )和 36例肝癌患者 (肝切除组 )肝切除手术前后血清TGF β1浓度 ;免疫组织化学法检测 36例肝癌和癌周组织标本中相关受体的表达情况。结果 肝癌患者血清TGF β1浓度较对照组显著升高 (P <0 .0 5 ) ;患者肝切除术后血清TGF β1浓度较术前有显著升高 (P <0 .0 1) ,而胆囊切除患者则无明显变化。癌组织与癌周组织受体表达的强度近似 ,但是部位有显著不同 ,36例癌周组织无论是否有肝硬化 ,受体染色均为阳性 ,以在细胞膜表面线形浓聚为特点 ,36例癌组织中仅 11例阳性 ,而且受体染色在细胞核周的胞浆中呈颗粒状浓聚 ,细胞膜表面则未发现受体染色。两者受体的染色阳性率差异有非常显著性 (P <0 .0 1)。结论 血清TGF β1水平可能是肝癌重要的血清标记物之一。肝叶切除能引起血清TGF β1水平的升高 ,但该因子受体只表达于癌细胞核周围的胞浆中 ,而在癌周组织中 ,则表达于细胞膜表面 ,提示癌细胞受TGF β1影响较小 ,故肝癌细胞的分裂增殖失控与TGF β1的抑制作用降低有关。
Objective\ To research the expression of TGF β 1 and its cell membrane receptors in patients with hepatocellular carcinoma. Methods\ ELISA was employed to determine the concentration of TGF β 1 in serum pre and post cholecystectomy of 27 patients (C group). The concentration of TGF β 1 in serum pre and post hepatectomy and the TGF β 1 receptors in liver tissue were determined in 36 patients with hepatocellular carcinoma (T group), respectively. Results\ The concentration of serum TGF β 1 in T group were higher than that in C group ( P <0.05). The post hepatectomy concentration of serum TGF β 1 in all patients were greatly higher than that of pre hepatectomy ( P <0.01), and the TGF β 1 receptor complex of normal liver tissue expressed on the cell membrane, but in tumoral tissue it was perinuclear accumulation rather than displayed membrane labeling. Conclusion\ TGF β 1 might be an important serum marker in patients with hepatoma. Hepatectomy can induce the enhancement of TGF β 1 in serum. It was presumed that the escape of tumoral hepatocytes from the control of TGF β 1 might be closely related to TGF β 1 receptors expressed in the cytoplasm perinuclear rather than on the cell membrane.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第6期535-536,共2页
Chinese Journal of Experimental Surgery
关键词
肝细胞癌
转化生长因子-Β1
受体
基因表达
Hepatocellular, carcinoma
\ Hepatectomy
\ Transforming growth factor β 1
\ Receptors
