摘要
目的:对黑曲霉Aspergillus niger xj抗菌活性及化学成分进行研究以筛选天然抗菌药物。方法:采用滤纸片扩散法对黑曲霉菌丝体石油醚、醋酸乙酯和水提取物进行抗菌活性测定,并对石油醚部位活性部位进行硅胶柱色谱,甲酯化,通过气相色谱-质谱联用技术(GC-MS)对其化学成分进行分析。结果:菌丝体石油醚部分具有一定抗菌活性,从中鉴定出10个化合物。结论:黑曲霉菌丝体的石油醚部分存在抗菌活性物质,其化学成分以脂肪酸占较大比例。
Objective：In order to develop natural antibacterial agents, the antibacterial activity of Aspergillus niger xj was investigated. Method： The disc diffusion method was used to measure the antibacterial activity of petroleum ether （P.E）, ethyl acetate （EtOAc） and water extracts of Aspergillus niger mycelia. The P.E fraction with antibacterial activity was separated by silica gel column chromatography method, then separated and identified by GC-MS after being esterified with methanol. Result： Ten compounds were isolated and identified from the petroleum ether extract of mycelia which exhibited inhibitory effect against Staphylococcus aureus. Conclusion： The petroleum ether extract of Aspergillus niger xj mycelia contained natural substances with antibacterial activity in which fatty acids are the main constituents.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期125-128,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
毕节地区马铃薯产业科技合作专项计划项目[毕署科(2010)04号]
贵州省农业攻关科技计划项目[黔科合NY字(2011)3053]
毕节循环经济研究院专项资金项目[毕循专合字(2010)ZK009号]
贵州省科学技术基金项目[黔科合J字(2010)2065号]
关键词
黑曲霉
菌丝体
抗菌活性
石油醚部分
GC-MS
Aspergillus niger
mycelia
antibacterial activity
petroleum ether fraction
GC-MS
