摘要
目的:建立同时测定鸡肉参中芦丁、槲皮苷、槲皮素含量的HPLC-DAD方法。方法:采用Diamonsil-C18色谱柱(150 mm×4.6 mm,5.0μm),柱温30℃,以甲醇(A)-0.5%磷酸水溶液(B)为流动相,进行梯度洗脱(0～5 min,48%～52%A;6～10 min,70%～30%A),检测波长254 nm,流速1.0 mL.min-1。结果:3种黄酮类成分在10 min内达到良好分离,芦丁、槲皮苷、槲皮素的线性范围分别为0.3～1.0 mg(r=0.999 8),0.030～0.10 mg(r=0.999 8),0.010～0.033 mg(r=0.999 5);平均加样回收率分别为100.95%(RSD 0.98%),99.51%(RSD 1.54%),102.84%(RSD 3.53%)。结论:该方法简便,快速,精确,可同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量,具有较好的重复性和稳定性,可作为鸡肉参药材的质量控制方法。
Objective：To establish an HPLC-DAD method for simultaneous determination of rutin, quercetin and quercitroside in Incarvillea mairei. Method： A Dikma Diamonsil C18column （4.6 mm×150 mm, 5 μm） was used. The column temperature was set at 30 ℃; the investigated compounds were separated with gradient mobile phase consisting of methanol （A）-0.5% phosphoric acid （B）; the flow rate was 1.0 mL·min-1 and the detection wavelength was kept at 254 nm. Result： The good separation of three flavonoids was achieved within 10 min. The linearity was achieved in the range of 0.30-1.0 mg （r=0.999 8） for rutin, 0.030-0.10 mg （r=0.999 8） for quercetin and 0.010-0.033 mg （r=0.999 5） for quercitrosid. The average recoveries were 100.95% （RSD 0.98%）, 99.51% （RSD 1.54%） and 102.84% （RSD 3.53%） respectively. Conclusion： The method is simple, rapid and accurate, which can be able to detect rutin, quercetin and quercitroside simultaneously, and can be used for quality control of I. mairei.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期120-122,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
大理市科技局科研基金资助项目(2008SF11)
关键词
HPLC
鸡肉参
芦丁
槲皮苷
槲皮素
HPLC
Incarvillea mairei
rutin
quercetin
quercitroside
