摘要
目的观察过氧化物酶体增殖子激活受体Y（pemxisomeproliferator-activatedreceptory，PPARl）激动剂罗格列酮对盲肠结扎穿孔（cecalligationpuncture，CLP）所致脓毒症大鼠肺损伤的影响。方法80只健康雄性Wistar大鼠，采用随机数字表法分为5组（每组16只）：假手术组（sHAM组）；盲肠结扎穿孔组（cLP组）；罗格列酮组（ROSI组），CLP前30min静脉注射罗格列酮0-3mg／kg；PPARY／拮抗剂GW9662组（GW组）：CLP前30rain静脉注射GW96620．3mg／kg；GW9662±罗格列酮组（GW±ROSI组）：依次于CLP前30min静脉注射GW96620．3ms／kg、CLP前15min静脉注射罗格列酮0．3ms／ks。于CLP后6、24h各组随机抽取8只大鼠处死并留取血液及肺组织。测定各组肺组织湿，干质量比（wet／dryratio，W／D）；肺组织髓过氧化物酶（myeloproxidase，MPO）活性；酶联免疫吸附法（enzymelinkedimmunosorbentassay，EusA）测定血浆肿瘤坏死因子咀（tumornecrosisfactor-or，TNF啾）水平；实时荧光定量聚合酶链反应（real-timePCR）测定肺组织高迁移率族蛋白Bl（highmobilitygroupproteinB1，HMGBl）mRNA水平。结果CLP组、ROSI组、GW组、GW±ROSI组大鼠W／D比、MPO活性、TNF-（x水平以及HMGBlmRNA水平在CLP后6、24h增加，均高于SHAM组。CLP后6h，CLP组、ROSI组、GW组、GW±ROSI组大鼠W／D比、MPO活性、TNF咄水平以及HMGBlmRNA水平分别为（5-31±0．14）、（3．15±0．47）U／g、（15．064±0．448）μg，L、（0．758±0．130）；（4．33±0．21）、（2．21±0．31）U／g、（3．334±0．212）斗g，L、（0．422±0．124）；（4．93±0．14）、（3．13±0．28）U／g、（15．001±0．343）μg，L、（0．732±0．198）；（4．87±0．13）、（3．08±0．35）U／g、（14．866±0．412）μg/L、（0．763±0．017），均高于SHAM组的（3．98±0．17）、（0．67±0．43）U/g（0．452±0．033）μg，L、（O．244i0．053）（P〈0．05）。CLP后24h，CLP组
Objective To observe the effects of rosiglitazone, an agonist of peroxisome proliferator-activated receptor , （PPARY）, on pulmonary injury in septic rats induced by cecal ligation puncture（CLP）. Methods Eighty healthy male Wistar rats were randomly divided into 5 groups： sham group （group SHAM）, cecal hgation puncture group （group CLP）. rosiglitazone group （group ROSI）, in which rats were intravenous injected with rosiglitazone 0.3 mg/kg 30 min before CLP. GW9662 group （group GW）, in which rats were intravenous injected with GW9662 0.3 mg/kg 30 rain before CLP. GW9662 plus rosiglitazone group （group GW±ROSI）, in which rats were injected intravenously with GW9662 0.3 mg/kg 30 min before CLP and then with rosiglitazone 0.3 mg/kg 15 min before CLP. Eight rats were sacrificed at 6 and 24 h after CLP respectively in each group. Plasma was collected for the measurement of the level of plasma tumor necrosis factor （TNF ） by enzyme linked immunosorbent assay （ELISA） and lung tissues were harvested for the detections of lung wet/dry （W/D） ratio, lung myeloperoxidase （MPO） activity, and lung tissue high mobility group protein B1 （HMGB1） mRNA expression by real-time PCR. Results The lung W/D ratio, MPO activity, plasma TNF-a level and the lung tissue HMGB1 mRNA expression of group CLP, group ROSI, group GW and group GW±ROSI were significantly higher than that of group SHAM. The lung W/D ratio, MPO activity, plasma TNF level and the lung tissue HMGB1mRNA expression of group CLP [（5.31±0.14）, （3.15±0.47） U/g, （15.064±0.448） μg/L and （0.758±0.130）1, group ROSI [（4.33± 0.21）, （2.21±0.31） U/g, （3.334±0.212） ±g/L and （0.422±0.124）1, group GW[（4.93±0.14）, （3.13±0.28） U/g, （15.001±0.343） I±g/L and （0.732±0.198） 1 and group GW±ROSI[ （4.87±0.13）, （3.08±0.35） U/g, （14.866±0.412） μg/L and （0.763±0.017） 1 at 6 h after CLP were significantly higher than that of the group SHAM [ （3.98±0.17）, （0.67
出处
《国际麻醉学与复苏杂志》
CAS
2013年第3期220-223,247,共5页
International Journal of Anesthesiology and Resuscitation
关键词
脓毒症
肺损伤
罗格列酮
Sepsis
Pulmonary injury
Rosiglitazone
