摘要
目的 探讨卵巢癌细胞MHC Ⅰ类分子表达与肿瘤微环境免疫活性细胞分布的关系 ,了解机体肿瘤微环境免疫功能状态。方法 用流式细胞仪检测卵巢癌细胞MHC Ⅰ类分子表达 ,同时用免疫组化方法检测肿瘤微环境中LCA+ 、CD3 + 细胞分布情况。结果 卵巢癌细胞MHC Ⅰ类分子表达存在不同程度的下降或缺如 ,且与肿瘤微环境中LCA+ 、CD3 + 细胞分布呈正相关 ,前者r=0 .846 ,P =0 .0 0 0 1,后者r =0 .738,P =0 .0 0 0 1。结论 上皮性卵巢癌细胞MHC Ⅰ类抗原分子的表达影响肿瘤微环境中浸润免疫细胞数 ,进而影响肿瘤微环境免疫监视能力。检测MHC Ⅰ类分子表达可为肿瘤浸润淋巴细胞临床过继治疗选择合适病例提供实验依据。
Purpose To determine the correlation between expression of MHC class Ⅰ on ovarian tumor cells and T cell infiltration in vivo on ovarian tumor micro environment. Methods Thirty three samples of primary epithelial ovarian carcers were analysed with flow cytometry for MHC class Ⅰ expression.Immunohistochemical analysis was performed using monoclonal antibodies that recognize leukocyte differentiation antigens(LCA +,CD 3 +) on cryostat sections of primary epithelial ovarian carcinoma.The results were compared with the expression of MHC class Ⅰexpression. Results Close correlation were revealed bewteen the expression of MHC class Ⅰ molecules and the numbers of infiltrating LCA + positive cells ( r=0.846,P=0.000 1) and CD 3 + cells ( r=0.738,P=0.000 1 ). Conclusions Expression of MHC class Ⅰ molecules may influence the status of human immunosurveillance in tumor micro environment on ovarian carcinoma and the assay may be helpful in selection patients for adoptive immnotherapy.
出处
《上海医科大学学报》
CSCD
2000年第3期201-203,207,F003,共5页
Journal of Fudan University(Medical Science)
