摘要
目的了解金黄色葡萄球菌、黏质沙雷菌和枯草芽孢杆菌芽孢的耐冲击能力和耐雾化能力的强弱,观察3种细菌的空气生物学特性。方法使用AGI-30型全玻璃液体冲击采样器(AGI-30)对3种细菌进行耐冲击实验,在12.5 L/min的气流冲击5、10和15 min后测定采样液中细菌滴度和终末采样液体积,采用校正存活率评价3种细菌的耐冲击性。用DV40型气溶胶发生器对3种细菌进行耐雾化实验,分别测定不同雾化时间后的菌液浓度,计算雾化后细菌存活率,评价3种细菌的耐雾化性能。结果采样冲击实验表明,金黄色葡萄球菌的存活率最高,但是在冲击过程中其存活率不稳定,枯草菌芽孢经过15 min的冲击后,存活率为60%,黏质沙雷菌的存活率为72%。在耐雾化能力方面,不同时间的雾化过程中,枯草菌芽孢的存活率最高,经过60 min的雾化后,存活率为83.56%;黏质沙雷菌的相对存活率为40%,金黄色葡萄球菌在雾化过程中存活率状态不稳定。结论金黄色葡萄球菌耐冲击能力最强,但存活率低、稳定性较差;枯草菌芽孢耐雾化能力最强,但耐冲击性能最差,且其特性不适宜作为生物气溶胶的模式菌。3种细菌中,黏质沙雷菌最适于作为生物安全装备生物学评价的指示微生物。
Objective To investigate the impinging resistance ability and the atomization resistance ability of Staphylo- coccus aureus, Serratia marcescens and spores of Bacillus subtilis and to observe their aerobiological characteristics. Methods All glass impinger（AGI-30） was used as the representative that would bubble during operation to complete the impinging experiment. The impinger was operated 5, 10 and 15 min at a flow rate of 12.5 L/min. The titers of bacteria and the vol- ume of final sampling solutions were determined before the colTected survival probability was used to evaluate the impinging resistance of the three named kinds of bacteria. DV40 was used in the atomization resistance experiment. The difference in atomization time and bacterial concentration was measured and the bacterial survival probability was calculated to evaluate the atomization resistance ability of the three kinds of bacteria. Results The sampling impinging tests showed that S. aureus had the highest survival rate, but its survival rate was unstable during impinging. After 15 min of impinging the survival rate of B. subtilis spores was 60% and that of S. marcescens was 72%, As for atomization capacity resistance, at different time of the atomization process, B. subtilis spores had the highest survival rate（83.56% after 60 min atomization） while that of S. marcescens was 40%. The S. aureus＇ survival in the atomization process was unstable. Conclusion S. aureus has the strongest impinging resistance, but the survival rate is less stable. The strongest atomization resistance ability goes to B. subtilis spores, but they have the worst impinging resistance and do not serve as a good biological aerosol model. The S. marcescens is the best indicator organism for biological evaluation of biosafety devices among the three kinds of bacteria.
出处
《中国社会工作》
2013年第5期30-32,42,共4页
China Social Work
基金
国家科技重大专项资助项目(2009ZX10004-703)
关键词
生物气溶胶
金黄色葡萄球菌
黏质沙雷茵
枯草芽孢杆菌
耐冲击性
耐雾化性
bioaerosols
Staphylococus aureus
Serratia marcescens
Bacillus subtilis
impinging resistance ability
atomization resistance ability
