摘要
采用 Bt可湿性粉剂标样和含单一 Bt杀虫蛋白 Cry A( c)的 MVPII水剂 ,研究改进了棉铃虫对 Bt杀虫蛋白的抗性测定方法。 1 996～ 1 998年进行华北地区棉铃虫对 Bt制剂的抗性监测 ,结果表明 ,河北省邱县、冀州市、山东省高密市、河南省西华县棉铃虫与敏感种群相比的抗性指数( RR)为 1 .3～ 5.3倍 ;用诊断剂量法测定 ,只在1 998年检测到山东高密棉铃虫的抗性个体频率为 0 .9% ,其余均为 0。 1 998年测定棉铃虫对Cry A( c)敏感性 ,与室内种群相比 ,河北邱县、冀州和山东高密棉铃虫种群对 Cry A( c)的相对 RR值分别为 4.9、3.9和 3.8倍 ,对 Bt粉剂的相对抗性指数 ( RR)分别为 1 .1、1 .4和 1 .6倍 ;用转 Bt基因棉叶直接测定 ,田间棉铃虫的死亡率降低 ,表明用 Cry A( c)进行测定与用转 Bt基因棉叶直接测定结果相关性较大。
Bioassay methods of Bt resistance in cotton bollworm,Helicoverpa armigera (Hübner) were studied. The results showed that the best time for investigating the mortality were 5 days after Bt treatment and 14 days after MVP treatment. Four field populations from three provinces of north China were collected in 1996~1998 for monitoring of resisance to Bacillus thuringiensis (Bt). The resistance ratios (RRs) to Bt formulation produced by Bt kurstaki were 1.3~5.3 folds in three years compared with a susceptible population from Xinjiang. Resistant individuals at 0.9% were found in 1998 in one population by using diagnostic dose. In 1998, the resistance grade of Bt cotton declined against Qiuxian,Jizhou and Gaomi populaions compared with a laboratory population. The relative RRs were 4.6,3.9,3.8 folds to CryⅠAc and 1.1,1.4,1.6 folds to Bt formulation, respectively. CryⅠAc was more suitable than Bt formulation to be used for resistance detection to Bt cotton.
出处
《棉花学报》
CSCD
北大核心
2000年第4期180-183,共4页
Cotton Science
基金
国家"8 63"课题资助!项目 ( Z1 7-0 2 -0 2 )
关键词
华北地区
棉铃虫
BT毒蛋白
抗药性
north China
Heliocoverpa armigera(Hübner)
Bt insecticidal proteins
resistance
