摘要
目的观察利拉鲁肽对糖耐量异常(IGT)OLETF大鼠血清糖基化终末产物(AGEs)、炎症指标及糖代谢的影响。方法实验共分为5组。12周龄IGT-OLETF大鼠32只,随机均分为4组,分别予生理盐水(OLETF-saline组)和不同剂量利拉鲁肽(50、100、200μg/kg)腹腔注射,2次/d;另取8只LETO大鼠注射生理盐水作为正常对照组。12周后测定各组大鼠血清AGEs、炎症因子及糖代谢指标水平。结果干预前IGT-OLETF大鼠空腹血糖(FPG)、餐后2h血糖(2hPG)、空腹胰岛素均较LETO大鼠升高;给予不同剂量利拉鲁肽干预12周后,FPG、2hPG、空腹胰岛素、AGEs及炎症指标肿瘤坏死因子-α(TNF-α)、血清超敏C反应蛋白(Hs-CRP)、白细胞介素-6(IL-6)水平均较OLETF-saline组降低(P<0.05)。干预实验结束后,OLETF-saline组87.5%的OLETF大鼠发展成糖尿病,利拉鲁肽干预组大鼠均未发生糖尿病。结论利拉鲁肽能有效改善IGT-OLETF大鼠的糖耐量异常及胰岛素抵抗,同时降低大鼠血清AGEs和炎症指标,提示利拉鲁肽可能通过降低非酶糖化和炎症水平而发挥抗炎作用,延缓IGT向糖尿病进展。
Objective To investigate the effect of liraglutide on serum advanced glycosylated end products （AGEs）, inflammatory factors and glycometabolism in OLETF rats with impaired glucose tolerance （IGT）. Methods Thirty-two 12-week- old OLETF rats were randomly assigned into 4 groups （8 each） and received intraperitoneal injection of saline （OLETF-saline group） or liraglutide 50, 100 or 2001xg/kg twice a day for 12 weeks （liraglutide intervention groups）. Eight LETO rats served as the normal control group and received saline injection. Twelve weeks after the treatment, all the rats were examined for fasting and 2h postprandial plasma glucose after Oral Glucose Tolerance Test （OGTT）, and serum AGEs and inflammatory factors were determined by enzyme linked immunosorbent assay （ELISA）. Results Before liraglutide intervention, the levels of fasting plasma glucose （FPG）, plasma glucose 2 hours after OGTT （2hPG）, and fasting plasma insulin （FPI） were significantly higher in IGT-OLETF rats than in LETO rats. While 12 weeks after liraglutide intervention, the levels of FPG, 2hPG, FPI, AGEs and the inflammatory factors TNF-cx, Hs-CRP and IL-6 declined obviously in IGT-OLETF rats compared with that in OLETF-saline rats （P〈0.05）. When the liraglutide intervention ended, 87.5% of the rats in OLETF-saline group developed diabetes, but no sign of diabetes was found in the rats of liraglutide intervention groups. Conclusion Liraglutide may effectively improve IGT and insulin-resistance, decrease the AGEs and inflammatory factors by inhibiting nonenzymatic glucosylation and inflammation to delay or prevent the development of diabetes in IGT-OLETF rats.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2013年第2期116-119,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金(81270966)
广东省自然科学基金(S2012010009494)
中华国际医学交流基金会资助项目(2100325)~~
