摘要
以戊二醛交联尼龙6膜载体固定化面包酵母DX213,采用固定化酵母细胞催化2-辛酮不对称还原得到(R)-2-辛醇。系统考察了有机溶剂、反应时间、pH、底物、辅助底物和热处理等因素对反应的产率和光学选择性的影响。结果表明,上述因素对酵母细胞催化不对称合成(R)-2-辛醇反应均有显著影响。二氯甲烷为该反应最适有机溶剂,在固定化细胞57 g/L(50℃预热50 min),水相与有机溶剂相体积比4/1,pH 7.0,初始2-辛酮浓度为60 mmoL/L(分别在反应0,10,17 h等分添加),蔗糖5.7 g/L和28℃条件下反应48 h,(R)-2-辛醇的产率和e.e.值分别达到89.3%和96.8%。
The baker yeast DX213 （Saccharomyces cerevisiae DX213 ） was immobilized on the activated woven nylon-6 membrane using glutaraldehyde as a cross-linking agent. In the process of asymmetric reduction of 2-octanone to 2-octanol by the immobilized yeast cells, the effect of organic solvent, reaction time, pH, 2- octanone, co-substrate and heating treatment on the yield and enantiomeric excess （e. e. value） of （R）-2-octanol was studied. The function mechanism of the influence factors with enzymatic activity of the ceils was presented in the reaction. The results showed that all the mentioned factors had effects on the reaction. Dichloromethane was found to be the optimal organic solvent for the reaction. Under the conditions of 57 g/L immobilized cell preheated at 50℃ for 50 min, 4/1 volume ratio of water phase to organic phase, pH7.0, 60 mmoL/L initial 2- octanone concentration added in three separate doses at 0, 10 and 27 h, 5.7 g/L sucrose concentration, 28℃ and reaction time 48 h, the yield and enantiomeric excess of （R） -2-octanol were 89. 3 % and 96. 8%, respectively.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第2期47-53,共7页
China Biotechnology
基金
湖南省科技厅社会发展支撑计划(2011SK3138)
湖南省研究生科研创新项目(CX2011B265)资助项目
