摘要
以种子发芽率和发芽势为种子萌发参数,以胚根、胚芽长度和鲜重变化为幼苗生长参数,研究了栗(Castanea mollissima)叶水提取物对双子叶植物莴苣(Lactuca sativa)、萝卜(Raphanus sativus)、黄瓜(Cucumis sativus),和单子叶植物洋葱(Allium cepa)、水稻(Oryza sativa)、小麦(Triticum aestivum)的化感作用;比较了美国板栗(Castanea dentata)与栗叶水提取物及经X-5大孔树脂分离获得的11个洗脱组分间的化感作用强弱;利用液相色谱-质谱联用技术结合标准物质的反证实验,解析了美国板栗与栗化感作用最强分离组分的物质结构。研究结果表明,栗叶化感效应强于美国板栗;美国板栗与栗化感作用最强分离组分中(5:5洗脱组分)存在绿原酸、对羟基苯甲酸、原儿茶酸和没食子酸等物质。在利用栗基因恢复美国板栗种群时,有必要考虑美国板栗与栗植物化学生态特性的差异。
Aims Castanea mollissima and C. dentata are typical chestnut species in Asia and North America, respectively. They play important roles in the ecology and economy in their existing forest ecosystems. Chestnut blight has caused catastrophic losses of C. dentata populations since 1904. It is especially important to understand the differences in chemical ecology characteristics between C. dentata and C. mollissima when scientists try to make use of blight resistance of C. dentata to help the recovery of C. mollissima populations in North America. The objective of this research is to compare the allelopathy effect of C. dentata and C. mollissima by leaf water ex-tract bioassay method. Methods Using germination rate and vigor as seed germination parameters and radicle, plumule elongation and fresh weight change of seedling as seedling growth parameters, we studied the effect of water extract of C. mol-lissima leaves on dicotyledons lettuce, radish and cucumber and monocotyledons onion, rice and wheat. We com-pared the allelopathic intensity of water extract and 11 elution components that were separated by X-5 macroporous resins from water extract of C. dentata and C. mollissima. The strongest allelopathic fraction of C. dentata and C. mollissima was analyzed by liquid chromatograph-mass spectrometer technique and standard substance counterevidence experiment. Important findings Evidence of inhibition of seed germination and seedling growth of test plants indicated that water extract of C. mollissima leaf has allelopathic properties. Data on inhibition of seed germination and seedling growth in lettuce indicated that the allelopathy of C. mollissima leaves was stronger than C. dentata leaves. The allelochemicals of C. dentata and C. mollissima extracts are in 7：3, 6：4 and 5：5 elution fractions, as documented by the bioassay data. The strongest allelopathy fraction of C. dentata and C. mollissima extract （5：5 elution fraction） contained chlorogenic acid, phydroxybenzoic acid, protocatechuic acid, gallic acid
出处
《植物生态学报》
CAS
CSCD
北大核心
2013年第2期173-182,共10页
Chinese Journal of Plant Ecology
基金
国家自然科学基金(30470334)
科技部星火重大项目(2012GA701002)
关键词
化感作用
栗属
液相色谱-质谱联用仪
种子萌发
实生苗生长
allelopathy
Castanea
liquid chromatographmass spectrometer
seed germination
seedling growth
