摘要
目的探讨乙肝肝硬化患者血清HBV-DNA的载量与肝纤维四项及单胺氧化酶之间的关系。方法对100例标本应用荧光定量PCR技术检测HBV-DNA;化学发光法检测该批标本血清中透明质酸(HA),层粘蛋白(LN),III型前胶质肽(PIIINP),IV型胶质(IV-C)均是肝纤维化标志物;同时检测血清中的单胺氧化酶。结果 100例活动性乙型肝炎肝硬化患者无论HBV-DNA水平如何,其肝纤维四项及单胺氧化酶均高于正常检测界限,在HBV高复制组(HBV-DNA>107拷贝/L)肝纤维化指标较低复制组(HBV-DNA<105拷贝/L)高,且差异具有统计学意义(P<0.05),而在HBV-DNA<105拷贝/L及105～107拷贝/L之间比较,其肝纤维化指标差异无统计学意义(P>0.05);而单胺氧化酶各组间差异均有统计学意义(P<0.05)。结论 HBV复制与肝纤维化血清学指标及单胺氧化酶存在相关性,但与肝纤维化血清学指标不呈直线相关,与单胺氧化酶呈直线相关趋势,因此对活动性乙型肝炎肝硬化患者抗病毒治疗非常重要,并联合检测纤维四项及单胺氧化酶可以提升肝纤维化临床诊断水平。
Objective To investigate the relationship among serum of HBV-DNA load and four indexes of liver fibrosis, MAO in the patients of HBV cirrhosis. Methods HBV-DNA of one hundred samples were exaimined with fluorescence quantitative PCR technique, four indexes of liver fibrosis one hundred samples were examined with radioimmunoassay, Meanwhile MAO index of serum was examined. Results No matter what HBV-DNA value was in one hundred active HBV cirrhosis patients,their four indexes of liver fibrosis and MAO are higher than normal examined result. Liver fibrosis index in HBV-DNA of high copy group（HBV-DNA≥10^7copy/L） was higher than that of HBV-DNA of low copy group （HBV-DNA≤10^5copy/L）, there were specific difference in them（P〈0. 05）. Whereas if HBV-DNA value was lower than 10^5 copy/L or in the range of 10^5- 10^7copy/L,liver fibrosis indexes had no significantly difference（P〉0. 05）. But MAO index exist remarkably difference （P〈0.05） in each group. Conclusion HBV copy was related with liver fibrosis level and MAO index. It was not linely related with liver fibrosis level, but its linely related with MAO index. The antiviral treatment was very important measure for the patients of active HBV cirrhosis,the clinical diagnostic level of liver fibrosis were improved by examing four indexes of liver fibrosis and MAO.
出处
《中国冶金工业医学杂志》
2013年第1期15-16,共2页
Chinese Medical Journal of Metallurgical industry
