摘要
作为模式生物,秀丽线虫(Caenorhabditis elegans)已经成功地用于许多生命过程的研究,尤其被广泛应用于现代发育生物学、行为与神经生物学、基因组学、正向和反向的遗传学研究中,近年来,秀丽线虫更成为了一个进行蛋白质组学研究的优良体系,诠释了基于基因组学和RNA干涉研究中的基因功能。许多比较蛋白质组学表达谱的建立可以更好地理解线虫在不同发育阶段、不同温度下基因的表达,在与人类神经疾病相关的疾病研究中,线虫对帕金森疾病、阿尔茨海默症、衰老与寿命、胰岛素通路都有所揭示。另外,线虫的亚蛋白质组学和翻译后修饰如糖基化和磷酸化也已经鉴定,其数据库也在不断地完善。本文介绍了秀丽线虫的蛋白质表达谱建立的历史,尤其是神经科学研究中的应用及翻译后修饰表达谱的建立等方面的研究现状,因此,结合其它分子生物学和基因工程技术,线虫蛋白质组学研究已成为提供一个新的全面的系统分析基因功能的重要工具,提示线虫是"蠕虫蛋白质组学"的一个丰富宝藏。
The nematode Caenorhabditis elegans_（C.elegans） has been used in many researches on biological processes.Although mostly known for its research value on modern developmental biology,behavior and neurobiology,genomics,powerful forward and reverse genetics,this model organism has developed into a respectable system for proteomics studies as well and used to complement genetic and RNA interference-based studies of gene function.A number of focused comparative studies contributed to a better understanding of differential gene expression under different temperatures and during development stages,revealed the mechanism of Parkinson,Alzheimer,aging and longevity,insulin signaling related to the human neuro-disease.In addition,C.elegans subproteomes and posttranslational modifications like glycosylation and phosphorylation have been identified and the database are endlessly consummate.Here we describe the history of C.elegans proteomics,especially in neuroscience,and the status of establishment of post-translation modification.Therefore,C.elegans proteomics,in combination of other molecular,biological and genetic techniques,would provide a versatile new tool box for the systematic analysis of gene functions.These studies suggest that C.elegans will be a rich trove for ＂worm proteomicists＂.
出处
《现代生物医学进展》
CAS
2012年第36期7183-7185,7095,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金青年基金项目(81100862)
关键词
秀丽线虫
蛋白质组学
翻译后修饰
Caenorhabditis elegans
Proteomics
Post-translation modification
