摘要
采用载玻片检测法,对碳氮源、温度、pH和光照等因子对孢子萌发与附着胞形成的影响进行研究。结果表明,供试碳氮源中,茶花炭疽菌孢子萌发的最佳碳源为葡萄糖、最佳氮源为酵母提取物;碳源对附着胞形成的影响不显著,而所有氮源均抑制附着胞的形成。孢子萌发和附着胞形成的最适温度同为20℃;最适pH为中性偏酸。孢子萌发的最佳光照条件为先进行12 h光照培养、再进行12 h黑暗处理;自然光条件下附着胞形成率最高。可见不同外界因子对茶花炭疽病病原菌孢子萌发和附着胞形成的影响不同,对该病害发病机理与侵染机制的研究,需要进一步结合致病性试验进行深入研究。
Effects of carbon and nitrogen sources, temperature, pH and illumination on conidium germination and appressorium formation were studied adopting concave slides. The results showed that among the tested carbon sources, the optimal one was glucose for camellia anthracnose conidium germination and the optimal nitrogen source was yeast extract. Carbon source had no significant impact on apprcssorium formation, but all nitrogen sources applied in studies inhibited the formation. The optimal temperature for conidium germination and appressorium formation was 20 ℃, pH was neutral to recta-acid. The optimal illumination condition for the conidium germination was 12 h of illumination followed by 12 h of darkness; under natural light condition, appressorium had the highest formation rate. It is thus clear that the effects of different external factors on conidium germination of camellia anthracnose had different effects on the germination of eonidium and appressorium formation. To elucidate the pathogenesis and infection mechanism of the disease it needs further study combined with pathogenic experiments to go into.
出处
《微生物学杂志》
CAS
CSCD
2012年第6期37-41,共5页
Journal of Microbiology
基金
浙江检验检疫局科技项目(ZK200619)
金华市科技项目(2006-1-27)
关键词
茶花
炭疽菌
孢子萌发
附着胞形成
影响因子
camellia
anthracnose
conidium germination
apprcssorium formation
affecting factor
