摘要
对影响番茄原生质体分离、培养以及再生植株的一些因素进行的初步研究表明:适合番茄原生质体分离、培养的材料是3～4周龄的无菌苗叶片;适合番茄原生质体培养的培养液渗透压以0.4 mol/L 甘露醇加山梨醇为好,培养液的量以1.5 ml/L 培养皿为适宜,选择合适的激素搭配浓度对维持愈伤组织的分化、抑制褐化是有效的;培养基中添加1.15mg/L NAA 适合诱导再生苗生根,无激素培养基适合再生植株的生长。
The results from the preliminary studies on the factors affecting tomato protoplast isolation,cultiure and plant regeneration were presented.The sutable materials for tomato protoplast isolation and culture were leaves from 3 to 4 week-old axenic seedlings.The osmotic pressure suitable for the protoplast cul- ture was 0.4mol/L mannitol anct sorbitol.The amount of culture medium suitable for the protoplast culture was 1.5 mL/petri dish.Suitable combination and concentra- tion of plant hormones were effecive to maintain the differentiation capacity and to inhibit the browning of the protoplast derived calluses.For the induction of roots from the regenerated stoots,the culture medium with 0.05mg/L NAA was suitable; for the growth of the regenerated plantlets,hormone free medium was stitable.
关键词
番茄
原生质体
再生植株
tomato
protoplast
plant regeneration
